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71 Cards in this Set
- Front
- Back
- 3rd side (hint)
What is Microbiology?
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-Study of life too small to be seen unaided by eye
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-using techniques of microbiology
-isolate microorganisms -study their characteristics |
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What is the visibility threshold of the human eye?
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-30-40 microns
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Who is Antoni van Leeuwenhoek?
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-Dutch tailor who began making and using simple microscopes
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-Father of Bacteriology and Protozoology
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Leeuwenhoek's microorganisms
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1. Fungi
2. Protozoa 3. Algae 4. Bacteria 5. Archaea 6. Small multicellular animals |
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Who is Carolus Linnaeus?
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-Swedish botanist who developed taxonomic system for naming plants and animals
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How can all living organisms be classified?
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-Eukaryotic
-Prokaryotic |
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What is a Eukaryote?
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-Organism that has a defined nucleus
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What is a Prokaryote?
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-Organism that does not have a defined nucleus
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Fungi are composed of what two subgroups?
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-Molds
-Yeasts |
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Describe Fungi
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- Relatively large microscopic eukaryotes
-Obtain food from other organisms |
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Describe Protozoa
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-Single-celled eukaryotes
-Similar to animals in nutrient needs and cellular structure. |
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How do protozoa move?
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-Pseudopodia
-Cilia -Flagella |
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Describe Algae
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-Unicellular or multicellular
-Plant like eukaryotes that are photosynthetic -categorized on the basis of pigment, storage product, and composition of cell wall |
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What are the two sub-groups of Algae?
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-Spirogyra
-Diatomes |
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Describe Bacteria and Archaea
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-Unicellular prokaryotes
-Live in a variety of communities and most habitats |
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What are the two kinds of Bacteria?
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-Bacteria
-Archaea |
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Describe Bacteria
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-Having cell walls that contain peptidoglycan; some lack cell walls.
-Most are beneficial, but some cause disease |
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Describe Archaea
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-Having cell walls composed of polymers other than peptidoglycan
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Fungi, Protozoa, Algae are?
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-Eukaryotes
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Bacteria and Archaea are?
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-Prokaryotes
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What was Francisco Redi's Experiment?
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-Meat in a jar
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What was John Needham's experiment?
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-Worked with gravy and plant infusions in sealed vials to see if microscopical animals would spontaneously generate
-Cloudy to clear and back to cloudy |
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Spallanzani concluded that
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-Needham failed to heat vials sufficiently
-microorganisms exist in air -Spontaneous generation does not occur |
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Who is Lewis Pasteur?
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-"Father of Microbiology"
-Swan neck flask experiment -Father of scientific method |
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Who is Eduard Buchner?
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-Demonstrated the presence of enzymes
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What are Enzymes?
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-Cell-produced proteins that promote chemical reactions such as fermentation.
-Proved that fermentation does not require living cells. |
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What is Metabolism?
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-The sum of all chemical reactions in an organism
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Pasteur developed what theory?
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-Germ theory of disease
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Define "Germ Theory of Disease"
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-Microorganisms are responsible for diseases
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Define Pathogen.
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-Microorganisms that cause specific diseases.
-Germ theory only applies to infectious diseases |
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Who is Robert Koch?
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-Studied Etiology
-Searched and discovered bacterium responsible for Anthrax -Developed techniques to isolate and examine colonies for microorganisms |
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Koch's Contributions
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-Simple straining techniques for bacterial cells and flagella
-First photo of bacteria/in diseased tissue -Techniques for estimating # of bacteria in a solution based on the number of colonies -use of steam to sterilize growth media -use of Petri dishes -techniques to transfer bacteria between media -elucidation of bacteria as distinct species |
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Define Koch's Postulates
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1. Suspected causative agent must be found in every case of the disease and be absent from healthy hosts
2. Agent must be isolated and grown outside the host 3 .Agent must be isolated and grown outside the host 4. Same agent must be found in the diseased experimental host |
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Ignaz Semmelweis
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-Hand washing to prevent nosocomial infections
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Who was Joseph Lister?
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-Antiseptic technique
-Sprayed wounds, surgical incisions, and dressings with carbolic acid which reduced deaths by two-thirds |
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Who was Florence Nightingale?
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-Nursing
-Introduced antiseptic techniques during the Crimean War |
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Who was John Snow?
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-Mapped the occurrence of cholera cases leading to foundation of two branches of microbiology: INFECTION CONTROL and EPIDEMIOLOGY
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What was Edward Jenner’s vaccine?
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-Pus from cowpox lesions prevented smallpox.
-Pasteur later developed vaccines for fowl cholera, anthrax, and rabies |
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What were Paul Ehrlich’s “magic bullets”?
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-Discovered chemicals effective against sleeping sickness and syphilis
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Leeuwenhoek founded what modern disciplines?
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-Bacteriology
-Protozoology -Mycology -Parasitology -Phycology |
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Linnaeus founded what modern discipline?
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-Taxonomy
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Semmelweiss and Snow founded what modern discipline?
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-Infection Control
-Epidemiology |
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Pasteur founded what modern discipline?
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-industrial microbiology
-Food and beverage technology (Pasteurization) |
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Buchner founded what modern discipline?
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-Microbial metabolism
-Genetics -Genetic engineering |
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Koch founded what modern discipline?
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-Etiology (Koch's Postulates)
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What is Etiology?
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-is the study of causation, or origination
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Ivanowski founded what modern discipline?
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-Virology
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Beijerinck and Winogradsky founded what modern disciplines?
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-Environmental microbiology
-Ecological microbiology |
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Gram founded what modern discipline?
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-Microbial morphology
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Lister and Nightingale founded what modern discipline?
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-Antiseptic medical techniques
-Hospital microbiology |
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Jenner founded what modern discipline?
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-Serology
-Immunology |
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Ehrlich founded what modern discipline?
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-Chemotherapy
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Fleming founded what modern discipline?
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-Pharmaceutical microbiology
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Define Biochemistry?
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-Study of Metabolism
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Kluyver and van Niel proposed what?
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-that biochemical reactions are shared by all living things, are few in number, and involve the transfer of electrons and hydrogen ions.
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Practical applications of Biochemistry
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-Design of herbicides and pesticides
-Diagnosis of illnesses and monitoring of patients’ responses to treatment -Treatment of metabolic diseases Drug design |
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Avery, MacLeod, and McCarty determined what?
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-Genes are contained in molecules of DNA (1940’s)
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Define Microbial Genetics
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-study of inheritance in microorganisms
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Beadle and Tatum established what?
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-that a gene’s activity is related to protein function
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Molecular Biology
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-Combines aspects of biochemistry, cell biology, and genetics
-Explanation of cell function at the molecular level |
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Pauling proposed what?
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-that gene sequences could:
1) Provide understanding of evolutionary relationships and processes 2) Establish taxonomic categories to reflect these relationships 3) Identify existence of microbes that have never been cultured |
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Woese determined what?
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-cells belong to bacteria, archaea, or eukaryotes
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Recombinant DNA Technology (Genetic Engineering)
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-Genes in microbes, plants, and animals manipulated for practical applications
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Define Gene Therapy
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-Use of recombinant DNA (DNA composed of genes from more than one organism) to insert a missing gene or repair a defective one in human cells
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Define Environmental microbiology
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-studies the role microorganisms play in their natural environment
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Define Biodegradation
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-Microbial communities play an essential role in the decay of dead organisms and recycling of chemicals such as carbon, nitrogen, and sulfur
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Define Bioremediation
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-uses living bacteria, fungi, and algae to detoxify polluted environments
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Von Behring and Kitasato discovered what?
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-the existence in the blood of chemicals and cells that fight infection
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Define Immunology
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-The study of the body’s defense against specific pathogens
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Fleming discovered what major drug?
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-Penicillin
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Domagk Discovered what group of drugs?
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-sulfa drugs
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