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71 Cards in this Set

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What is Microbiology?
-Study of life too small to be seen unaided by eye
-using techniques of microbiology
-isolate microorganisms
-study their characteristics
What is the visibility threshold of the human eye?
-30-40 microns
Who is Antoni van Leeuwenhoek?
-Dutch tailor who began making and using simple microscopes
-Father of Bacteriology and Protozoology
Leeuwenhoek's microorganisms
1. Fungi
2. Protozoa
3. Algae
4. Bacteria
5. Archaea
6. Small multicellular animals
Who is Carolus Linnaeus?
-Swedish botanist who developed taxonomic system for naming plants and animals
How can all living organisms be classified?
-Eukaryotic
-Prokaryotic
What is a Eukaryote?
-Organism that has a defined nucleus
What is a Prokaryote?
-Organism that does not have a defined nucleus
Fungi are composed of what two subgroups?
-Molds
-Yeasts
Describe Fungi
- Relatively large microscopic eukaryotes
-Obtain food from other organisms
Describe Protozoa
-Single-celled eukaryotes
-Similar to animals in nutrient needs and cellular structure.
How do protozoa move?
-Pseudopodia
-Cilia
-Flagella
Describe Algae
-Unicellular or multicellular
-Plant like eukaryotes that are photosynthetic
-categorized on the basis of pigment, storage product, and composition of cell wall
What are the two sub-groups of Algae?
-Spirogyra
-Diatomes
Describe Bacteria and Archaea
-Unicellular prokaryotes
-Live in a variety of communities and most habitats
What are the two kinds of Bacteria?
-Bacteria
-Archaea
Describe Bacteria
-Having cell walls that contain peptidoglycan; some lack cell walls.
-Most are beneficial, but some cause disease
Describe Archaea
-Having cell walls composed of polymers other than peptidoglycan
Fungi, Protozoa, Algae are?
-Eukaryotes
Bacteria and Archaea are?
-Prokaryotes
What was Francisco Redi's Experiment?
-Meat in a jar
What was John Needham's experiment?
-Worked with gravy and plant infusions in sealed vials to see if microscopical animals would spontaneously generate
-Cloudy to clear and back to cloudy
Spallanzani concluded that
-Needham failed to heat vials sufficiently
-microorganisms exist in air
-Spontaneous generation does not occur
Who is Lewis Pasteur?
-"Father of Microbiology"
-Swan neck flask experiment
-Father of scientific method
Who is Eduard Buchner?
-Demonstrated the presence of enzymes
What are Enzymes?
-Cell-produced proteins that promote chemical reactions such as fermentation.
-Proved that fermentation does not require living cells.
What is Metabolism?
-The sum of all chemical reactions in an organism
Pasteur developed what theory?
-Germ theory of disease
Define "Germ Theory of Disease"
-Microorganisms are responsible for diseases
Define Pathogen.
-Microorganisms that cause specific diseases.
-Germ theory only applies to infectious diseases
Who is Robert Koch?
-Studied Etiology
-Searched and discovered bacterium responsible for Anthrax
-Developed techniques to isolate and examine colonies for microorganisms
Koch's Contributions
-Simple straining techniques for bacterial cells and flagella
-First photo of bacteria/in diseased tissue
-Techniques for estimating # of bacteria in a solution based on the number of colonies
-use of steam to sterilize growth media
-use of Petri dishes
-techniques to transfer bacteria between media
-elucidation of bacteria as distinct species
Define Koch's Postulates
1. Suspected causative agent must be found in every case of the disease and be absent from healthy hosts
2. Agent must be isolated and grown outside the host
3 .Agent must be isolated and grown outside the host
4. Same agent must be found in the diseased experimental host
Ignaz Semmelweis
-Hand washing to prevent nosocomial infections
Who was Joseph Lister?
-Antiseptic technique
-Sprayed wounds, surgical incisions, and dressings with carbolic acid which reduced deaths by two-thirds
Who was Florence Nightingale?
-Nursing
-Introduced antiseptic techniques during the Crimean War
Who was John Snow?
-Mapped the occurrence of cholera cases leading to foundation of two branches of microbiology: INFECTION CONTROL and EPIDEMIOLOGY
What was Edward Jenner’s vaccine?
-Pus from cowpox lesions prevented smallpox.
-Pasteur later developed vaccines for fowl cholera, anthrax, and rabies
What were Paul Ehrlich’s “magic bullets”?
-Discovered chemicals effective against sleeping sickness and syphilis
Leeuwenhoek founded what modern disciplines?
-Bacteriology
-Protozoology
-Mycology
-Parasitology
-Phycology
Linnaeus founded what modern discipline?
-Taxonomy
Semmelweiss and Snow founded what modern discipline?
-Infection Control
-Epidemiology
Pasteur founded what modern discipline?
-industrial microbiology
-Food and beverage technology (Pasteurization)
Buchner founded what modern discipline?
-Microbial metabolism
-Genetics
-Genetic engineering
Koch founded what modern discipline?
-Etiology (Koch's Postulates)
What is Etiology?
-is the study of causation, or origination
Ivanowski founded what modern discipline?
-Virology
Beijerinck and Winogradsky founded what modern disciplines?
-Environmental microbiology
-Ecological microbiology
Gram founded what modern discipline?
-Microbial morphology
Lister and Nightingale founded what modern discipline?
-Antiseptic medical techniques
-Hospital microbiology
Jenner founded what modern discipline?
-Serology
-Immunology
Ehrlich founded what modern discipline?
-Chemotherapy
Fleming founded what modern discipline?
-Pharmaceutical microbiology
Define Biochemistry?
-Study of Metabolism
Kluyver and van Niel proposed what?
-that biochemical reactions are shared by all living things, are few in number, and involve the transfer of electrons and hydrogen ions.
Practical applications of Biochemistry
-Design of herbicides and pesticides
-Diagnosis of illnesses and monitoring of patients’ responses to treatment
-Treatment of metabolic diseases
Drug design
Avery, MacLeod, and McCarty determined what?
-Genes are contained in molecules of DNA (1940’s)
Define Microbial Genetics
-study of inheritance in microorganisms
Beadle and Tatum established what?
-that a gene’s activity is related to protein function
Molecular Biology
-Combines aspects of biochemistry, cell biology, and genetics
-Explanation of cell function at the molecular level
Pauling proposed what?
-that gene sequences could:
1) Provide understanding of evolutionary relationships and processes
2) Establish taxonomic categories to reflect these relationships
3) Identify existence of microbes that have never been cultured
Woese determined what?
-cells belong to bacteria, archaea, or eukaryotes
Recombinant DNA Technology (Genetic Engineering)
-Genes in microbes, plants, and animals manipulated for practical applications
Define Gene Therapy
-Use of recombinant DNA (DNA composed of genes from more than one organism) to insert a missing gene or repair a defective one in human cells
Define Environmental microbiology
-studies the role microorganisms play in their natural environment
Define Biodegradation
-Microbial communities play an essential role in the decay of dead organisms and recycling of chemicals such as carbon, nitrogen, and sulfur
Define Bioremediation
-uses living bacteria, fungi, and algae to detoxify polluted environments
Von Behring and Kitasato discovered what?
-the existence in the blood of chemicals and cells that fight infection
Define Immunology
-The study of the body’s defense against specific pathogens
Fleming discovered what major drug?
-Penicillin
Domagk Discovered what group of drugs?
-sulfa drugs