Bio 209 Unit 2

Front 1

Starch Hydrolysis Test

Back 1

Test: Starch agar (NA+starch) is used. Gram's iodine is added to the area of growth.
Positive: Hydrolyzed starch produces a clear zone from the production of amylase.
Negative: Starch in the presence of iodine yields a blue-black color.

Front 2

Starch Hydrolysis

Back 2

Starch is a glucose polymer linked together by glycosidic bonds.

Front 3

Lipid Hydrolysis

Back 3

Positive: Organisms which produce esterases will generate a cleared zone surrounding the area of growth when grown on Tributyrin Agar plates. If grown on Spirit Blue Agar, the growth will have a darker blue border.
Negative: No production, medium is unchanged. (This is true for broth media).

Front 4

Lipid Hydrolysis

Back 4

Triglycerides are an example of lipids with high amounts of energy essential to cell membrane.

Front 5

Protein Hydrolysis

Back 5

Test: Organisms grown on milk agar (NA+milk).
Positive: Produce a clear zone when hydrolysis has occurred.
Negative: Remains opaque without hydrolysis.

Front 6

Protein hydrolysis

Back 6

Caesin is a milk protein composed of amino acids linked together by peptide bonds. Before assimilation into the cell, proteins must be broken down step by step to their basic units, amino acids. Breakdown occurs through the process, proteolysis or peptonization.

Front 7

Gelatin Hydrolysis

Back 7

Can be rapid or slow. Cultures that remained liquefied when chilled after incubation are organisms that produce gelatinase.

Front 8

Gelatin Hydrolysis

Back 8

Gelatin is a protein produced by hydrolysis of collagen. It exists as a liquid above 25 degrees C. Below 25 degrees C, it is a gel.