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23 Cards in this Set
- Front
- Back
What is the % of DNA expressed in the eukaryotic genome at any one time?
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1%
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Do muscle cells and nerve cells have different genetic information?
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No
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What is the function of methylated DNA?
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Methylated DNA has an extra methane that prevents it from being transcribed
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In prokaryotic cells, how does the primary transcript compare to processed mRNA?
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They're both functional
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Moving a part of one chromosome to another non-homologous chromosome is called?
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Translocation
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Can certain genes increase in number in a cell? Can they be lost?
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Gene amplification. Yes, in deletion.
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What kind of hormone can have a direct effect of promoting transcription?
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Steroid hormones
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List two things that pseudogenes lack.
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p. 359
-Doesn't yield functinoal products -Lack promoter region for transcription and doesn't get transcribed |
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What is unique sequence DNA?
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Only one copy of it
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What are chromosomal puffs and what is their significance?
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Transcription
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List four different ways in which a proto-oncogene can be converted into an oncogene.
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1. Translocation
2. Move promotor region in front of oncogene 3. Mutation - makes it active 4. Gene amplification |
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What is gene therapy?
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a healthy gene substituted for an unhealthy one
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Is gene therapy being done on somatic or germ cells?
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Somatic
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What is the function of polymerase chain reaction?
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To allow synthesis of primary copies on DNA
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What enzyme is used in the polymerase chain reaction?
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DNA polymerates
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How much growth was on the LB/Amp- petri dish? Why?
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A lot, there was no ampicillan
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How much growth was on the LB/Amp+ petri dish? Why?
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None or little
none: didn't pick up plasmid little: did pick up plasmid |
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What are the two major steps in creating a DNA fingerprint.
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Cutting out DNA with restriction enzymes.
Use electrophloreses |
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What is a cloning vector? Give two examples.
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Used to insert a gene:
1. DNA gun 2. Plasmid transformation 3. Direct insertion 4. Using virus to carry gene in 5. TI plasmid |
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What is the purpose of gel electrophoresis?
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To separate DNA by size
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Why do scientist like to use yeast cells in genetic experiments?
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Recombinant DNA - transformation - screen and foam
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In making recombinant DNA, what is the problem with using two different restriction enzymes?
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Don't have matching sticky ends
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Fragments of DNA that have been separated of gel electrophoresis, may or may not have a desired gene. How can the appropriate ones be identified?
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Filter paper.. use radioactive probes with single stranded DNA
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