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11 Cards in this Set
- Front
- Back
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What happens to DNA synthesis in the presence of ddNTPs?
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chain termination occurs
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True or False: Sequencing uses fluorescently labeled ddNTPs
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true
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What is used to separate DNA molecules?
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Gel electrophoresis
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What moves the fastest, short or long DNA? and Why?
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Short moves fastest because it can squeeze through other molecules because of its size versus a large DNA
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Why does DNA move towards the positive electrode?
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Because, it is negatively charged. Therefore, opposite charges attract one another.
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What is a gene?
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a gene is the entire DNA sequence needed to make a functional gene product. i.e. RNA.
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How do we know which parts of the genome is transcribed into RNA?
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first you Isolate RNA from cells, then analyze by hybridization techniques (for ex. Northern blotting), lastly analyze by sequencing (sanger or high-throughput)
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True or False: genes do not encode protein.
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False, some genes encode protein
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What are some examples of non-coding RNAs and their functions?
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1. rRNAs- protein synthesis
2. tRNAs- protein synthesis 3. snRNAs- mRNA splicing 4. snoRNAs- Pre-rRNA processing and rRNA modification 5. miRNAs- regulation of gene expression 6. Telomerase RNA- Template for addition of telomeres |
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What is the difference between solitary vs. duplicated genes?
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Solitary genes are found only once in the genome while duplicated genes are multiple genes of close but usually not identical sequences.
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What is an example of a duplicated gene?
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1. The human B-globin gene family:
2. rRNA genes |
