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19 Cards in this Set
- Front
- Back
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What does Millon test for? |
Specific for phenol group of amino acid Tyrosine. |
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How do you perform a Millon test? |
5ml of unknown & 3 to 4 drops of Millon Reagent Heat |
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How do you know a Millon test is postivie? |
Red color & coagulum |
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What does Biuret test for? |
2 amino groups united by peptide bond CONH2 |
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How do you perform Biuret test? |
1ml of unknown & 4 to 5 drops of Biuret Reagent |
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How do you know if Biuret test is positive? |
Appearance of pink to purple |
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What does Benedict's test for? |
Tests for aldehyde or ketone groups of reducing sugars |
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How do you perform Benedict's test? |
10 drops of unknown 5ml of Benedict's solution Heat to boiling for 2 minutes |
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How do you know if Benedict's test is positive? |
Green-Red/Brown is positive. Indicates glucose if after first test & sucrose after second test. |
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What will a Benedict's test react positive for? |
Monosaccharides: glucose, reducing sugars |
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If Benedict's test has no reaction (blue color), what should you do next? |
Take a new tube & put 10 drops of the unknown with 1ml of HCI. Heat for 2 minutes, cool, then add 5ml of Benedict's solution. Heat again for 2 minutes. |
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What will the second Benedict's test do? |
Break down a disaccharide to monosaccharides which will test positive. |
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What does iodine test for? |
Starch |
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How do you tell if an iodine test is positive? |
Blue-black color. |
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How do you perform an iodine test? |
Test a few drops of the unknown with a few drops of iodine. |
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What is starch? |
A polysaccharide |
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What are two characteristics of lipids? |
Insoluble in water & soluble in fat solvents |
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How do you test for lipids? |
Add unknown to water & see if it separates on top of the watery layer. |
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What tests do you not need to perform for unknown? |
Sudan dye or grease spot. |
