Bi 331 7-1

Front 1

Immediately after birth we gain waht

Back 1

Internal and external bacterial flora that constantly fluctuating in number and species and remain with us throughout our lifetimes

Front 2

What does the skin serve as

Back 2

An effective barrier against microbial invasion

Front 3

What does the skin contain

Back 3

Resident populations

Front 4

Are resident populations generally harmless

Back 4

Yes, they may even provide some protective capacity

Front 5

Why do transient species appear?

Back 5

As a result of contact with the surroundings

Front 6

Are trasnient species variable

Back 6

Yes, they are highly variable

Front 7

Can transient species be washed off easily

Back 7

Yes

Front 8

Where do resident species live

Back 8

In or on the skin

Front 9

What do the resident species use as nutrient sources

Back 9

Skin secretions and wastes

Front 10

What is the exercise trying to enumerate

Back 10

2 groups of bacterial flora

Promionibacterium

Micrococcaeae

Front 11

What are the different types of skin using

Back 11

Dry, moist, oily

Front 12

Can this procedure recorver 100% of the resident bacterial cells

Back 12

No

Due to their inaccessibility

Front 13

What does the donor do

Back 13

Bacterial flora will be sampled from them

Front 14

Should donors currnetly be using a topical anti-bacterial product?

Back 14

No

Front 15

Is there a simple relationship between personal hygiene and density and type of skin flora?

Back 15

No

Front 16

The surface to be sampled is _____

Back 16

Horizontal

Front 17

How long will each sample take

Back 17

2 minutes

Front 18

How to do a wash

Back 18

Place a sterile sampling cylinder on the area to e sampled and apply only enough pressure to ensure no leakage of the wash fluid.

Pipette 1 ml of wash fluid into the cylinder. Rub the skin for 1 minute with a sterile glass rod

Endeavor to rub the entire area of skin enclosed by the cylinder

Withdraw the wash fluid with a pipette and dispense into an empty sterile test tube

Front 19

How many washes should you do of the same area

Back 19

Two and then pool the two wash samples

Front 20

Why do we draw up the fluid with a 5 ml pipette

Back 20

To estimate the total volume

Front 21

How much of the dilutions should we spread onto the plates

Back 21

0.1 ml

Front 22

What are the two media we are using

Back 22

RCMF

HBA

Front 23

Is RCMF incubated anaerobically or aerbocially

Back 23

Anaerobically

Front 24

What does RCMF select for

Back 24

Propionibacterium

Front 25

HBA is what

Back 25

Heated blood agar

Front 26

Is HBA incubated aerbocially or anaerobically

Back 26

Aerobically

Front 27

What does HBA allow for the growth of

Back 27

Micrococcaceae

Front 28

Where do we place the RCMF plates

Back 28

In an anerobic jar

Front 29

How do we incubate HBA?

Back 29

Aerobically

Front 30

What do we do when we first examine the HBA and RCMF plates

Back 30

count and note the different colony types

Front 31

What do we do after we count and note the different colony types

Back 31

Perform gram stains from represetnative colonies

Front 32

Will we perform diagnostic tests for identification

Back 32

No

Front 33

What are aerobic flora

Back 33

Gram positive cocci

Often in clusters but not chains

Colonies are white, gray, golden, yellow, hemolytic or non-hemolytic

Front 34

What are anaerobic flora

Back 34

Gram-positive rod or club shaped cells

Often clumped in groups resembling picket fences

Colonies are brown-yellow, pink and mucoid or pink and dry with scalloped edge

Front 35

When doing hte calculation what must we take into consideration

Back 35

Dilution used