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68 Cards in this Set
- Front
- Back
Brain Heart Infusion (BHI) |
Medium of choice for culturing the yeast form of dimorphic fungi |
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Lactophenol Cotton Blue |
Most useful stain/emulsifying agent for fungi |
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Germ Tube Test |
Most basic and easiest test for demonstration of yeast form of fungi (Candida albicans) |
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Saborauds Dextrose Agar |
Best culture medium for isolation of fungi |
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Bronchial Washings |
Best specimen for the isolation of L. pneumophilia |
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Nasopharyngeal swab |
Best specimens for the isolation of Bordetella pertussis |
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25 to 30 degrees celcius |
Optimal temperature for the isolation of Mycobacterium marinum |
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4 degrees celcius |
Optimal temperature for the storage of specimensfor the purpose of isolating viruses |
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Refrigerator Temperature |
Ideal temp to hold a fecal specimen |
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1.18 |
Specific gravity of the approved zinc sulfate for flotation method |
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Charcoal Cephalexin Agar |
Best culture media for the isolation of B. pertussis |
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Human embryonic fibroblast agar |
Best culture media for the isolation of cytomegalovirus |
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Campy Blood Agar |
Best culture media for the isolation of Salmonella enteritidis |
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Buffered Charcoal Yeast Agar/Extract |
Best culture media for the isolation of Legionella pneumophilia |
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Human Blood Tween |
Best culture media for the isolation of Gardnerella vaginalis |
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India (China) Ink |
Routinely used to examine cultures of Cryptococcus Neoformans |
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Giemsa stain/Wright's stain |
Excellent stain for the yeast cells of Histoplasma capsulatum in tissue or blood smears |
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Methylene Blue stain |
Stain used mainly to see the etiologic agent of tinea versicolor |
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Periodic Acid Schiff |
One of the best stains for visualizing fungi in scrapings or in tissues |
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Seller's Stain |
Most effective for staining cells of Cryptococcus neoformans in smears of infected brain tissue |
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Chlamydospore (Cornmeal) Agar |
Used worldwide to identify Candida albicans |
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Hair Baiting |
Used to isolate many dermatophytes |
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Formalin-ether Sedimentation |
Preferred concentrated method for the isolation of operculated eggs |
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Inulin Clearance Test |
Gold standard for clearance Test |
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Kjeldahl |
Standard reference method of protein based on nitrogen |
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Hexokinase |
The best enzymatic method for glucose determination |
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O-toluidine |
The best non-enzymatic method for glucose determination |
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8.6 to10 |
Optimal ph for Alkaline Phosphatase |
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4.9 to 5 |
Optimal pH for Acid Phosphatase |
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6.9 to 7 |
Optimal pH for Amylase |
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7.8 to 8 |
Optimal pH for Lipase |
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7.1 |
Optimal ph for AST and ALT |
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9 |
Optimal pH for CK forward reaction |
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6.8 |
Optimal pH for CK backward reaction |
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6.8 to 7 |
Optimal pH for Aldolase |
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API 20C |
Gold standard for carbohydrate assimilation testing of yeast |
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Lloyd's method |
Reference method for Creatinine |
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Autoclave |
Best method of sterilization |
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Cyanmethemoglobin |
Best method of hemoglobin determination |
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Mueller Hinton Agar (MHA) |
Recommendedmedium for antimicrobial susceptibility testing |
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0.5 McFarland Standard |
Standard used in adjusting the turbidity of suspension of test organism |
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Serum |
The most appropriate clinical specimen for the assay of antimicrobial agents |
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Coagulase Test |
Best single criterion of pathogenicity of Staphylococcus aureus |
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First Morning |
Preferred urine specimen for cytology |
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Peroxidase stain |
Recommended stain for demonstration of Auer rods |
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10% formol saline |
Recommended fixative for central nervous tissues and general postmortem tissues |
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Neutral Buffered Formalin |
Recommended for preservation and storage of surgical, postmortem, and research specimens; best fixative for demonstration of elastic fibre and iron pigments |
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Formol-corrosive (Formol sublimate) Formol Mercuric Chloride Solution |
Recommended fixative for routine post mortem tissues |
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Mercuric Chloride |
Fixative of choice for preservation of cell detail in tissue photography and is recommended for renal tissues, fibrin connective tissues and muscles |
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Zenker's fluid (mercuric chloride plus glacial acetic acid to be added just before use) |
Recommended for fixing small pieces of liver, spleen, connective tissue fibres, and nuclei |
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Helly's Solution (Zenker-Formol) (Mercuric chloride plus 40% formaldehyde to be added just before use) |
An excellent micro anatomic fixative for pituitary gland, bone marrow and blood containing organs such as spleen and liver |
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Heidenhain's Susa Solution |
Recommended for tumor biopsies especially of the skin and excellent cytologic fixative |
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Regaud's (Moller's) Fluid |
Recommended for demonstration of chromatin, mitochondria, mitotic figures, golgi bodies, RBC and colloid containing tissues |
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Orth's fluid |
Recommended for study of early degenerative process and tissue necrosis; it demonstrates Rickettsiae and other bacteria |
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Bouin's Solution |
Recommended for fixation of embryos |
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Carnoy's fluid |
Recommended for fixing chromosomes lymph glands and urgent biopsies (fastest fixative: 1-3 hours) |
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Newcomer's fluid |
Recommended for fixing you mucopolysaccharides and nuclear proteins |
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Acetone |
Recommended for the study of water diffusible enzymes especially phosphatases and lipases |
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Bone marrow preparation |
Best specimen for demonstration of ferric iron |
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Afternoon specimens (2-4) |
Ideal specimen for urobilinogen determination |
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Sodium polyethanol sulfonate (SPS) |
Best anticoagulant for blood culture |
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Sweat chloride |
Best index for cystic fibrosis of the pancreas |
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Throat culture |
Gold standard for streptococcal infection in children |
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Citrates |
Anticoagulant of choice for blood coagulation studies |
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Heparin |
Anticoagulant of choice for ABG and open heart surgery |
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EDTA |
Anticoagulant of choice for cellular morphology |
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Fluorides |
Anticoagulant of choice for glucose determination in which the sample is to be transported or whenever analysis is to be delayed |
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Balanced oxalate |
Anticoagulant of choice in ESR determination |