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68 Cards in this Set

  • Front
  • Back

Brain Heart Infusion (BHI)

Medium of choice for culturing the yeast form of dimorphic fungi

Lactophenol Cotton Blue

Most useful stain/emulsifying agent for fungi

Germ Tube Test

Most basic and easiest test for demonstration of yeast form of fungi (Candida albicans)

Saborauds Dextrose Agar

Best culture medium for isolation of fungi

Bronchial Washings

Best specimen for the isolation of L. pneumophilia

Nasopharyngeal swab

Best specimens for the isolation of Bordetella pertussis

25 to 30 degrees celcius

Optimal temperature for the isolation of Mycobacterium marinum

4 degrees celcius

Optimal temperature for the storage of specimensfor the purpose of isolating viruses

Refrigerator Temperature

Ideal temp to hold a fecal specimen

1.18

Specific gravity of the approved zinc sulfate for flotation method

Charcoal Cephalexin Agar

Best culture media for the isolation of B. pertussis

Human embryonic fibroblast agar

Best culture media for the isolation of cytomegalovirus

Campy Blood Agar

Best culture media for the isolation of Salmonella enteritidis

Buffered Charcoal Yeast Agar/Extract

Best culture media for the isolation of Legionella pneumophilia

Human Blood Tween

Best culture media for the isolation of Gardnerella vaginalis

India (China) Ink

Routinely used to examine cultures of Cryptococcus Neoformans

Giemsa stain/Wright's stain

Excellent stain for the yeast cells of Histoplasma capsulatum in tissue or blood smears

Methylene Blue stain

Stain used mainly to see the etiologic agent of tinea versicolor

Periodic Acid Schiff

One of the best stains for visualizing fungi in scrapings or in tissues

Seller's Stain

Most effective for staining cells of Cryptococcus neoformans in smears of infected brain tissue

Chlamydospore (Cornmeal) Agar

Used worldwide to identify Candida albicans

Hair Baiting

Used to isolate many dermatophytes

Formalin-ether Sedimentation

Preferred concentrated method for the isolation of operculated eggs

Inulin Clearance Test

Gold standard for clearance Test

Kjeldahl

Standard reference method of protein based on nitrogen

Hexokinase

The best enzymatic method for glucose determination

O-toluidine

The best non-enzymatic method for glucose determination

8.6 to10

Optimal ph for Alkaline Phosphatase

4.9 to 5

Optimal pH for Acid Phosphatase

6.9 to 7

Optimal pH for Amylase

7.8 to 8

Optimal pH for Lipase

7.1

Optimal ph for AST and ALT

9

Optimal pH for CK forward reaction

6.8

Optimal pH for CK backward reaction

6.8 to 7

Optimal pH for Aldolase

API 20C

Gold standard for carbohydrate assimilation testing of yeast

Lloyd's method

Reference method for Creatinine

Autoclave

Best method of sterilization

Cyanmethemoglobin

Best method of hemoglobin determination

Mueller Hinton Agar (MHA)

Recommendedmedium for antimicrobial susceptibility testing

0.5 McFarland Standard

Standard used in adjusting the turbidity of suspension of test organism

Serum

The most appropriate clinical specimen for the assay of antimicrobial agents

Coagulase Test

Best single criterion of pathogenicity of Staphylococcus aureus

First Morning

Preferred urine specimen for cytology

Peroxidase stain

Recommended stain for demonstration of Auer rods

10% formol saline

Recommended fixative for central nervous tissues and general postmortem tissues

Neutral Buffered Formalin

Recommended for preservation and storage of surgical, postmortem, and research specimens; best fixative for demonstration of elastic fibre and iron pigments

Formol-corrosive (Formol sublimate) Formol Mercuric Chloride Solution

Recommended fixative for routine post mortem tissues

Mercuric Chloride

Fixative of choice for preservation of cell detail in tissue photography and is recommended for renal tissues, fibrin connective tissues and muscles

Zenker's fluid (mercuric chloride plus glacial acetic acid to be added just before use)

Recommended for fixing small pieces of liver, spleen, connective tissue fibres, and nuclei

Helly's Solution (Zenker-Formol) (Mercuric chloride plus 40% formaldehyde to be added just before use)

An excellent micro anatomic fixative for pituitary gland, bone marrow and blood containing organs such as spleen and liver

Heidenhain's Susa Solution

Recommended for tumor biopsies especially of the skin and excellent cytologic fixative

Regaud's (Moller's) Fluid

Recommended for demonstration of chromatin, mitochondria, mitotic figures, golgi bodies, RBC and colloid containing tissues

Orth's fluid

Recommended for study of early degenerative process and tissue necrosis; it demonstrates Rickettsiae and other bacteria

Bouin's Solution

Recommended for fixation of embryos

Carnoy's fluid

Recommended for fixing chromosomes lymph glands and urgent biopsies (fastest fixative: 1-3 hours)

Newcomer's fluid

Recommended for fixing you mucopolysaccharides and nuclear proteins

Acetone

Recommended for the study of water diffusible enzymes especially phosphatases and lipases

Bone marrow preparation

Best specimen for demonstration of ferric iron

Afternoon specimens (2-4)

Ideal specimen for urobilinogen determination

Sodium polyethanol sulfonate (SPS)

Best anticoagulant for blood culture

Sweat chloride

Best index for cystic fibrosis of the pancreas

Throat culture

Gold standard for streptococcal infection in children

Citrates

Anticoagulant of choice for blood coagulation studies

Heparin

Anticoagulant of choice for ABG and open heart surgery

EDTA

Anticoagulant of choice for cellular morphology

Fluorides

Anticoagulant of choice for glucose determination in which the sample is to be transported or whenever analysis is to be delayed

Balanced oxalate

Anticoagulant of choice in ESR determination