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16 Cards in this Set

  • Front
  • Back
Sterilization
free of any living organisms.
Cultural Media
containing the necessary nutrients are used to grow microorganisms in a laboratory.
Synthetic media
known amounts of pure chemical
Non synthetic media
complex materials rich in vitamins and nutrients composition is unknown
Broth
A liquid that contains nutrients for bacteria to grow .
Best way to hold the test tubes from the..
Base or bottom
Agar plates
Petri dishes that contain agar for growing microorganisms.
What has a large surface area and are useful for isolating and styding microorganism
Agar plates
Agar slants
useful for perserving cultures
Agar deep tubes
useful for styduing gas requirements of microbes.
Why the plates are flipped??
1. Condensation droplets will fall on the top… we don’t want it on the culture(medium); prevent condensation droplets.
2. Prevent contamination.
Subculturing
transferring microorganisms from one medium to another.
What is this an example of:bacteria growing in broth may be transferred to an agar plate.
subculturing
Wire loops
are used to transfer microorganisms from liquid media to liquid or solid media.
Needles
are used for transferring microorganisms to deep tubes.
Pipettes
are used to transfer liquids. A mechanical device must be used with pipettes to create a vacuum.