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20 Cards in this Set

  • Front
  • Back
Examples of ssDNA phages
phiX-174, M13
ssDNA mechanism
converted to dsDNA by host DNA polymerase to make replicative intermediate

M13 is secreted from cell
RNA phage mechanism
Codes for RNA replicase, enzyme to replicate RNA genome

Capsid proteins made and ssRNA is packaged
RNA phage example and characteristics
MS2 - small, tailless, phages of E. coli, only 3-4 genes
dsRNA phage example
Phi-6 - infects Pseudomonas
dsDNA mechanism
Lysogenic - stays in the host cell until INDUCTION
dsDNA example
Lambda phage
viral genome of temperate phage
Temperate phage
capable of lysogeny
switching to lytic cycle
Mechanism of Lytic or Lysogenic decision?
cl and cro compete for same binding site on phage DNA

cl = represses synthesis of all genes --> lysogenic

cro = represses synthesis of cl --> lytic
When bacteria isn't growing well, it enters which cycle?
Lysogenic because small amount being made at low level
Lysogenic conversion
change induced in host phenotype by presence of prophage due to expression of additional genes from prophage
Applications of bacteriophages
Alternative treatment for bacterial infections because self-replicating, smart drugs and narrow specificity
enzyme that pokes holes
enzyme that breaks host cell wall peptidoglycan
Examples of lytic and lysogenic dsDNA
lytic = T4
lysogenic = lambda
Classification of bacteriophages
Morphology - Head shape, contractile, noncontractile tails, tailless, filamentous

nucleic acid properties

dsDNA, ssDNA, ssRNA, dsRNA
What receptor sites are there for phages on bacteria?
T-even: proteins, lipopolysaccharides

Teichoic acids, cell wall components
Sex pilus
Lytic Life Cycle steps
1) Adsorption into host cell and penetration
2) Synthesis of phage nucleic acids and proteins
3) Phage DNA is replicated
4) Assembly of phage particles
5) Release of phage particles