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30 Cards in this Set
- Front
- Back
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What are the essential nutrients for bacteria? (5)
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C,N,energy,H20,Ions
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What 2 pathways do bacteria use for sources of energy?
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Fermintation
Respiration |
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What is another name for Embden-Meyerhof-Parnas pathway?
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Glycolytic pathway
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Fermintation (3)
(Anaerobic) |
1) Organic acid and alcohol production.
2) low ATP, no ETC 3) o2 is not the final e- acceptor |
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Respiration (3)
(Aerobic) |
1) o2 is present
2) TCA, ETC 3) High ATP production |
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Facultative Anaerobic Bacteria
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These bacteria utilize both pathways for energy production
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What are 4 ways bacteria can be studied?
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light microscope
Gram stain Acid fast stain Fluorescent Microscopy |
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What are some properties of Agar? (4)
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1) Polysaccharide extracted from algae
2) Dissolves @ 100 3 Solidifies @ 45 4) most common culture prep. Good for identifying |
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What are the 3 types of media?
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Nutrient
Selective Differential/Indicator |
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Nutrient media (2)
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1) Contains essential ingredients for bac growth
2) Milk, beans, vit, salt |
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Selective media (2)
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1) inhibits growth of unwanted organisms
2) contains antimicrobial agents |
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Differential/Indicator media (2)
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1) shows biochemical features of the organism
2) carbs and pH added to see if the organism ferments it |
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Microaerophilic
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02 required, but less than what is found in air. It likes high C02
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Catalase
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H202 >>>>> H20 + o2
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Carbohydrate Breakdown
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test ability of organism to produce metabolic products from breaking down sugars
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Urease Production
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tests for hydrolysis of Urea to produce CO2 & NH4, this is detected by ^pH
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Coagulase
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Fibrinogen >>> Fibrin
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Oxidase production
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Testing for cytochrome C which can oxidize a reagent
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Voges-Proskauer test
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detects products of glucose fermintation to Acetoin
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Hydrogen sulfide test
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test ability of organism to produce H2S
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Indole test
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tests for indole (derived from tryptophan)
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Binary fission steps (4)
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1) chromosome division
2) cell lengthened, chromosomes segregated 3) cross wall complete 4) daughter cells seperate |
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What are the 4 phases of bacterial growth?
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Lag
Log Stationary Death (Decline) |
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Lag phase
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cells increase in size, no binary fission
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Log phase
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binary fission occurs
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Stationary phase
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1) cell growth=cell death
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Death (decline) phase
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no division
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Facultative organisms (3)
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1) ferment only
2) ferment or respire 3) respire only |
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Obligate anaerobes or strict anaerobes
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1) ferments
OR 2) ferments and respires. The final e- acceptor can be something other that o2 (fumarate) |
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Strict or obligate aerobes (1)
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1) Respires only
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