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39 Cards in this Set
- Front
- Back
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Consist of circular piece of double stranded DNA (dsDNA) that is supercoiled to fit in inside the cell |
Bacterial chromosome (genome) |
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Bacterial chromosome(genome) |
Contains all the info necessary for cell growth and replication |
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Also known as bacterial culture |
Bacterial cultivation |
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Is any material containing the necessary nutritional and environmental requirements for bacterial growth |
Culture medium |
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Classification of culture media |
*Accdg to physical state or consistency *Accdg to composition *Accdg to Manner of dispensing *Accdg to function or Use |
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Accdg to Physical state or consistency |
*liquid culture media *semi-solid culture media *solid culture media |
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Accdg to composition |
*synthetic *non-synthetic *tissue culture media |
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Accdg to Manner of dispensing or distribution |
*Plated *tubed |
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Accdg to function or Use |
*simple or supportive *enrichment *enriched *differential media *selective media *special media *transport media *media for antimicrobial susceptibility testing *media for biochemical tests |
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Lactose fermentation |
*rapid lactose fermenters *late lactose fermenters *non lactose fermenter *hydrogen sulfide (H2S) producers |
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Without any trace of agar or any solidifying agent |
Liquid culture media |
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Examples of liquid culture media |
*nutrient broth *thioglycollate broth |
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Contains 0.5-1.0% agar |
Semi-solid culture media |
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Examples of Semi-solid culture media |
*SIM (Sulfide indole motility) |
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Contains 2-3% agar |
Solid culture media |
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Examples of Solid culture media |
*Luria agar *blood agar plate *nutrient agar, etc. |
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Accdg to composition: chemically defined. The exact amt. Of the chemical composition of the ingredients are known |
Synthetic |
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Example of synthetic |
Commercially prepared culture media |
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Accdg to composition: Complex. The precise composition of some or all nutrients used were not known |
Non-synthetic |
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Examples of non-synthetic |
Meat extract broth/ animal and plant extracts |
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Accdg to composition: contains living tissues used for growing microorganism that cannot grow on cell free or artificially prepared culture media |
Tissue culture media |
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Examples of Tissue culture media |
*Embryonated egg- for viruses *Mc Coy- for Chlamydia *M. Leprae- armadillo/ mice |
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Manner of dispensing/ distribution |
Plated |
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Examples of plated media |
BAP, CAP, MaC, MHA, etc. |
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Distributed in sterile test tubes |
Tubed |
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Types of tubed media |
*slant *butt *butt-slant |
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Examples of tubed media |
(TSI) triple sugar iron agar (LIA) lysine iron agar (SCA) Simmon's citrase agar (SIM) sulfide indole motility Urea Agar |
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General/ primary isolation media; supports the growth of non-fastidious organisms for routine cultivation |
Simple or supportive |
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Examples of simple/ supportive media |
(NA) nutrient agar (NB) nutrient broth (TSA) trypticase soy agar (TSB) trypticase soy broth Luna agar/ broth |
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Designed to enhance the growth of particular organisms, usually pathogens, and spores the growth of normal flora present in the specimen |
Enrichment media |
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Examples of enrichment media |
*alkaline peptone water- vibrio *selenite broth/ tetrathionate broth- salmonella & shigella |
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Supports the growth of fastidious organisms |
EnRiched media |
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Example of enriched media |
CAP and BAP |
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CAP- chocolate agar plate Added with Isovitalex for the growth of: |
*Streptococcus pneumoniae *Haemophilus influenzae *Neisseria species |
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CAP is heated at: |
80°C |
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Uses 5-10% defibrinated blood |
BAP- Blood Agar Plate |
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BAP- Blood Agar Plate preferred blood |
Sheep's blood- most preferred Horse blood (2°) Rabbit's blood (3°) Human blood (4°) |
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Human blood is the least preferred because of some non-specific inhibitors: |
*Citrate- inhibits growth of B-hemolytic Streptococci *Dextrose- alters the type of hemolysis |
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Distinguishes organisms growing together by their differences in cultural characteristics |
Differential media |
