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19 Cards in this Set
- Front
- Back
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Classical Identification of prokaryotes is based on?
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morphology
biochemistry Goal: rapid diagnosis of pathogens |
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molecular identification of bacteria is based on?
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relationship among bacteria are reflected in their DNA
hybridization to DNA/RNA amplifiacation of DNA |
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what are types of molecular diagnositic dna analysis
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1. hybridizations
2. amplification of pathogen specific DNA (PCR) 3. analysis of restriction endonuclease patterns (RFLP) |
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Strength of bond btwn and target in in situ hybridization order is?
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1. RNA - RNA
2. RNA- DNA 3. DNA - DNA |
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Aerobes
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metabolize O2
grow only in its presence |
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Microaerophiles
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metabolize O2
grow only in low O2 |
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Facultative anaerobes
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metabolize O2 when present
ferment when no O2 |
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Anaerobes
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dont metabolize O2 but ferment
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Aerotolerant
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dont metab O2 but ferment in the presence or absence of O2
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Facultative anaerobes use what as the terminal oxidase
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cytochorome d
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strict aerobes use waht as the terminal oxidase?
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cytochrome c
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End product of fermentation of yeast
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ethanol
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End product of mixed acid fermentation of enterobacteria
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acetylaldehyde
lactate acetate formate some ethanol |
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Under acidic conditions enterocytes formate converted by hydrogenase to gas is + in? - in?
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+: E. Coli and Salmonella
-: Shigella and S. Typhi |
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If you suspect water contamination you do a VP test to see if it is sewage. What do the results mean?
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+: Enterobacteria from veg
-: E.coli from sewage |
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What enzyme causes gase to be made in acidic conditons?
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formate hydrolyase
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Which of these are gm +? -?
Staph. aureus E. coli |
+ Staph
- E. Coli |
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What 3 bacteria use sporulation?
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Bacillus
Clostridium Sporosarcina |
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How do you inactivate a spore?
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autoclave -- they are heat resistant
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