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19 Cards in this Set

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  • Back
Classical Identification of prokaryotes is based on?
morphology
biochemistry

Goal: rapid diagnosis of pathogens
molecular identification of bacteria is based on?
relationship among bacteria are reflected in their DNA

hybridization to DNA/RNA
amplifiacation of DNA
what are types of molecular diagnositic dna analysis
1. hybridizations
2. amplification of pathogen specific DNA (PCR)
3. analysis of restriction endonuclease patterns (RFLP)
Strength of bond btwn and target in in situ hybridization order is?
1. RNA - RNA
2. RNA- DNA
3. DNA - DNA
Aerobes
metabolize O2
grow only in its presence
Microaerophiles
metabolize O2
grow only in low O2
Facultative anaerobes
metabolize O2 when present
ferment when no O2
Anaerobes
dont metabolize O2 but ferment
Aerotolerant
dont metab O2 but ferment in the presence or absence of O2
Facultative anaerobes use what as the terminal oxidase
cytochorome d
strict aerobes use waht as the terminal oxidase?
cytochrome c
End product of fermentation of yeast
ethanol
End product of mixed acid fermentation of enterobacteria
acetylaldehyde
lactate
acetate
formate
some ethanol
Under acidic conditions enterocytes formate converted by hydrogenase to gas is + in? - in?
+: E. Coli and Salmonella
-: Shigella and S. Typhi
If you suspect water contamination you do a VP test to see if it is sewage. What do the results mean?
+: Enterobacteria from veg
-: E.coli from sewage
What enzyme causes gase to be made in acidic conditons?
formate hydrolyase
Which of these are gm +? -?
Staph. aureus
E. coli
+ Staph
- E. Coli
What 3 bacteria use sporulation?
Bacillus
Clostridium
Sporosarcina
How do you inactivate a spore?
autoclave -- they are heat resistant