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118 Cards in this Set
- Front
- Back
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What are one of the differences between B. anthracis and B.Cereus?
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B.anthracis- not B. hemolytic
not motile B,cereys - B hemolyic motile |
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Which organism has tumbling motility?
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Listeria monocytogenes
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Which organism has an umbrella motility stab
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Listeria monocytogenes
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What is the only gram + rod that produces H2S (TSI slant)
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Erysipelothrix thusiopathiae
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Whick gram - rod is lactose and indole positive?
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E.coli
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Which organism has a green sheen on EMB agar?
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E.coli
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Which organism has a mucoid colony and currant jelly sputum?
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Klebsiella pneumoniae
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Which organism has swarming motility?
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Proteus
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Which proteus is indole positive?
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Proteus vulgaris
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Which proteus is indole negative?
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Proteus mirabilis
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Which gram - bacillus has red pigment?
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Serratia marcesencs
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Which gram - rods are encapsulated?
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Klebsiella and Salmonella
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Which gram - rod use the serlologic "Kaufman White typing scheme"?
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Salmonella
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What are the O somatic antigens, H flagellar antigens and Vi Capular antigens and which organism are the found on?
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Salmonella
-O somatic antigen - cell wall -H flagellar antigen - 2 phases h1 and h2 -Vi capuslar antigen - found ony in S. Typhi |
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Which salmonella antigen is only found on S. Typhi?
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Vi capsular antigen
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What special processing does salmonella typhi need for processing?
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Need to boil solution for 15 minutes to destroy the Vi antigen and expose the D somatic antigen.
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Which organism gives a mustache of H2S on TSI tube?
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Salmonella typhi
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Which gram - organism is non moitle and produes no H2S
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Shigella
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**Which shigella has group D?**
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S. Sonnei
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Which shigella has group B?
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S.flexneri
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Which shigella has group A?
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S dysenteriae
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Which shigella has group C
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S, boydii
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Which non-Listeria organism grows well at 4*C?
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Yersinia enteroclitica
-has been associated with banked blood infections |
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Which gram - bacilli is associated with septicemia in iron overload sydromes?
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Yersinia enterocolitica
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Which gram - bacillli is associated with mesenteric adenitis
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Yersinia enterocolitica
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Which gram - organism has a bipolar staining "safety pin" appearance?
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Yersinia pestis
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What causes the rice water stools in vibrio cholera?
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Toxin that activates adenylate cyclase-inc CAMP, which leads to hypersecretion of NaCl and water
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What does Yersinia pestis grow on and what are its biochemical characteristics?
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Grows on BAP,
-catalase + -oxidase - |
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Which gram - organism is associated with a positive "string test"?
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Vibrio cholera
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What selective media would you use to grow Vibrio Cholera?
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TCBS-turns yellow (sucrose positive)
-is halophilic, 1% salt enhances growth |
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What are the toxins produced by vibrio cholera?
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-O1- most virulent
-El Tor |
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Which vibrio is lactose positive
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Vulnificans, which is highly virulent.
Ingesion of raw oysters and shellfish |
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Oxidative/Fermentative sugar reactions
2 tubes containing glucose (green indicator), 1 with oil, 1 without oil. What type if both turn yellow? |
Fermentation
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Oxidative/Fermentative sugar reactions
2 tubes containing glucose (green indicator), 1 with oil, 1 without oil. What type if only tube without oil turns yellow? |
Assmilation
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Oxidative/Fermentative sugar reactions
2 tubes containing glucose (green indicator), 1 with oil, 1 without oil. What type if no tubes turn yellow? |
Oxidizer
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On TCBS agar, what organism turns from yellow suggesing sucrose positivity?
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Vibrio cholera
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Which vibrio species is sucrose negative - green on TCBS?
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Vibrio parahemolyticus
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Which vibrio can you get from raw oysters and shellfish?
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Vibrio vulnficans- highly virulent with muscle necrosis and septicimia with 50% fatalality.
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Which vibrio is lactose positive?
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Vibrio vulnificans- green or yellow on TCBS
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Which pseudomonas species grows at 42 *C?
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Pseudomonas aeruginous grows at 42*C.
Putida does not grow at 42*C |
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What is the glucose and oxidase status of Pseudomonas?
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-Glucose non-fermenter
-Oxidase + |
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Which glucose non-fermenter has a sweet fruity odor?
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Alkaligenes
-nosocomial/environment |
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What glucose non fermenting organism causes fatal septicemia in neonates?
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Flavobacterium (Chryseobacterium)
-F. meningosepticum |
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What is a fastidious gram negative rod?
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Haemophilus
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What is haemophilus grown on?
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Chocolate agar?
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Which organism satellites off of Staph aureus?
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Heamophilus
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What is V?
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NAD
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What is X?
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heme
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Which organisms have a Quellung reaction? What is a Quellung reaction?
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H. influenza
St. pneumonia -Capsular swelling from specific capsular antibody |
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What factors does H. influenza require?
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V and X
+ Quellung reaction |
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What factors does H. hemolyticus require?
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V and X
B-hemolytic |
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What factors does H. parainfluenza erequire?
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V
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What factors does H. parahemolyticus require?
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V
B-hemolytic |
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What factors does H. ducreyi require?
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X
Associated with chancroid |
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Which hemophilus require no V or X?
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H. aphrophilus
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What organisms are in the HACEK group?
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Haemophilas aphrophilus
Actinomcyetecomitans Cardiobacterium hominis Eikenella corrodens Kingella kingii |
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Which HACEK organism group is:
oxidase - catalase- |
Haemophilus aphrophilus
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Which HACEK organism group is:
oxidase - catalase + |
Actinobacillus actinomycetecomitans
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Which HACEK organism group is:
oxidase + |
Cardiobecterium hominis
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Which HACEK organism group is:
oxidase + pits BAP |
Eikinella corrodens
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Which HACEK organism group is
oxidase + hemolytic on BAP: |
Kingella kingii
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What media is used to trasnsport and culture Bordetella pertussis?
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Regan Lower Charcoal agar
-Use a nasopharyngeal swab for culture |
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What methods can be used to detect Bortella?
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Fluorescent antibody stain
PCR |
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What are the three stages of Bordetella pertusis?
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1. prodromal ----- most infectious
2. catarrhal 3. paroxysmal |
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What organisms are associated with dor or cat bites?
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Pateurella multicoda
Capnocytophyga canimorsis - very pleomorphic gram - rod |
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Does Pasteurella multocida grow on MacConkey agar?
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No, it will grow on blood agar.
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What is a chemical feature of Pasteurella multocida?
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Oxidase +
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How do you treat P. multocida?
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Treat with penicillin
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What is Brucella cultured on?
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Castaeda biphasic blood agar = hold for 14-21 days.
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Where is Brucella found?
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Found in blood and bone marrow
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What is Brucella abortus accociated with?
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raw cow milk
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What is Brucella melitensis associated with?
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raw goat milk, feta cheese
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Wahat is Brucella suis associated with
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pigs
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What is Brucella cannis associated with?
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dogs
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What temperatures does Campylobacter jejuni grow at?
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37*C and 42*C (likes high temperatures)
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What temperature does C.fetus grow at ?
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37*C and 25*C (likes low temperatures)
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What organism has gliding motility and fingerlike projections from its colonies?
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Captnocytophagia
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Which gram - rod is fusiform and very pleomorhpic
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Captnocytophagia
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What are the chemical characteristics of Captnophagia?
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Oxidase -
Catalase - |
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What amino acid does Francisella require for culture?
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Cysteine
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What culture media does Legionella need to grow in?
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Buffered Charcoal Yeast Extract Agar (BCYE)
-requires cysteine for growth |
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How does legionella stain?
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Does not gram stain.
-use silver impregnation -or use carbol fuschin instread of safranin as a counterstain |
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What tests are available for Legionalla?
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Urine antibody test.
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What do you treat Legionella with?
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Erythromycin
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Which bateria have no cell walls?
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Myocplasma
Ureaplasma |
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What special media must ureaplasma and mycoplasma have?
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Sterols
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What bacteria has fried egg colony vaginitis, cervicitis, postpartum sepsis, neonatal infections, pre reupture of membranes?
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Mycoplasma hominis
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Which bacteria are difficult or unable to grow?
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Bartonella bacilliformis
Bartonella quintana Chlamydia trachomatis Chlamydia penuomoniae Chlamydia psittaci Erlichia E. chaffeensis Erlichia |
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What is associated with cat scratch fever, bacillary angiomatosis and Oroyo Fever?
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Bartonella bacilliformis
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What is associated with trench fever?
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Bartonella quintana
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What is associated with lymphogranuloma venereum?
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Chlamydia trachomatis-serovars L1, L2, L3
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What causes replasing fever and what is the insect vector?
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Borrelia recurrentis
Human body louse |
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What are some important anaerobic bacteria?
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Bacteroirdes fragilis
Fusobacterium Clostridium |
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What are the main metabolic features of Bacteroides Fragilis?
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-Grows in bile
-Esculin + Main organism in human bowel. Non fragilis groups are sensitive to bile |
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Which bacteroides is indole positive?
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B. thetaiotamicrons
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What gram negative bacteria has a spindle shape and pointed ends?
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Fusobacterium
-Associated with mouth and respiratory tract abscess |
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Which gram + bacillus has a double zone of hemolysis?
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Clostridium perfringens
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Which gram + bacillus has a reverse camp + test?
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Clostridium perfringens
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Which clostriudium has a tennis racket look appearance?
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Clostridium tetani
-tennis racket looking organism due to spore at the end. |
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Which Clostridium is associated with cancer?
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Clostrium speticum
-other bacteria is Strep bovis |
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Which organism has bread crumb colonies and molar tooth colonies on agar plates?
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Actinomyces
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Which anaerobic gram positive rod can cause cerebral shunt infections and shunt nephritis?
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Proprionobacterium acnes
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Associations:
tularemia |
Francisella tularensis
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Associations:
pertussis |
Bordetella pertussis
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Associations:
chancroid |
Haemophilus ducreyi
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Associations:
dogn bite |
Pasteurella multocida
Capnophyga canimorsus |
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Associations:
Legionnaire's |
Legionella pneumophila
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Associations:
Bacterial Vaginosis |
Gardnerella vaginalis
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Associations:
*****Granuloma inguinale |
Calymnobacterium granulomatis
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Associations:
rat bite fever |
Streptobacillus moniliformis
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Associations:
Lyme disease |
Borrelia burgdorferi
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Associations:
Borreliosis |
B. recurrentis
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Associations:
erysipeloid |
Erysipelothrix rhusiopathiae
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Associations:
ersipelas |
Beta Strep group A
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Which bacteria are H2S +?
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Salmonella
Proteus Citrobacter Edwardsiella Erysiplothrix (only Gram +) |
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Which bacteria are motile?
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Salmonella
Proteus Burkholderia Vibrio |
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What is BBL Chromagar used for?
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Distinguishes E. coli o157 (mauve colonies) from E.coli non0157(blue colonies). Also inhibits Proteus, Pseudomonas and Auromonas.
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Which Enterobacterobacteriaceae are cytocrhome oxidase negative?
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They are all negative.
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