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41 Cards in this Set

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5 areas of bacterial identification
morphology
metabolism
specific macromolecules (O-antigens; exoproteins; capsular antigens)
other characteristics (motility; spore formation)
DNA/RNA analysis
3 possibilities for energy metabolism
obligate aerobe
obligate anaerobe
facultative organism
respiration vs. fermentation
respiration uses oxygen and produces 26 ATP
fermentation doesn't use oxygen and produces 4 ATP
name some specific macromolecules used in identification
O antigens
K antigens
H antigens
capsule; pilus; flagellum
polysaccharides
cell wall antigens
what is biotyping?
identification of bacteria by analysis of sugar metabolism; presence of specific proteins, lipases, or nucleases
2 groups of filamentous bacteria
Actinomyces
Mycobacteria (cause TB and leprosy)
characteristics of filamentous bacteria
originally thought to be fungi
rods
stain poorly with gram stain
4 groups of obligate intracellular bacteria
Rickettsia
Chlamydia
Coxiella
Erlichia
what are obligate intracellular bacteria obligate intracellular organisms?
they have lost their cellular machinery and therefore their ability to generate ATP
Rickettsia
obligate intracellular
Chalmydia
obligate intracellular
Coxiella
obligate intracellular
Erlichia
obligate intracellular
spirochetes: characteristics and name 3
difficult to culture
lack LPS but have lipoproteins
Treponema - syphilis
Borellia - Lyme disease
Leptospira
Leptospira
spirochete
Treponema
syphilis (spirochete)
Borellia
Lyme disease (spirochete)
name 4 groups of gram positive cocci
facultative organisms:
Staph
Strep
Pneumococci

obligate anaerobes:
Peptostreptococci
Staphylococci
gram positive cocci
facultative
Streptococci
gram positive cocci
facultative
Pneumococci
gram positive cocci
facultative
Peptostreptococci
gram postive cocci
obligate anaerobes
4 groups of gram positive rods
large pleomorphs:
Clostridia
Bacilli

small pleomorphs:
Listeria
Corynebacteria
gram positive organisms (rods and cocci)
Staph; Strep; Pneumococci; Peptostreptococci

Corynebacteria; Listeria; Bacilli; Clostridia
Corynebacteria
gram positive rod
small pleomorph
Listeria
gram positive rod
small pleomorph
Clostridia
gram positive rod
large pleomorph
obligate anaerobe
Bacilli
gram positive rod
large pleomorph
facultative
3 major groups of gram negative organisms
curved rods; straight rods; small fastidious rods
curved rods (gram negatives)
aerobes:
Vibrio
Campylobacter

anaerobes:
Spirillum
Spirillum
gram negative curved rod
anaerobe
Vibrio
gram negative curved rod
aerobe
Campylobacter
gram negative curved rod
aerobe
small fastidious rods (gram negatives)
(6 groups)
Hemophilus
Bordatella
Pasturella
Yersinia
Franicella
Brucella
Brucella
small fastidious gram negative rod
Pasturella
small fastidious gram negative rod
Franicella
small fastidious gram negative rod
Bordatella
small fastidious gram negative rod
Yersinia
small fastidious gram negative rod
Hemophilus
small fastidious gram negative rod
what makes an organism fastidious?
it has lost part of its genome and therefore can't make essential metabolic products on its own - has highly specific nutritional requirements