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7 Cards in this Set

  • Front
  • Back

Acetamide Utilization

Principle: use acetamide as sole of carbon, deaminate acetamide to release ammonia


pH-driven color: green to royal blue


pos rxn: blue color


neg rxn: no color change

Acetate Utilization

Principle: breakdown of sodium acetate to shift toward the alkaline range


indicator: from green to blue


pos rxn: medium becomes alkaline blue


neg rxn: no growth or growth w/ no indicator change to blue

Bacitracin Test

organism: Streptococcus pyogenes (inhibited by the small amount of bacitracin in disk)


pos rxn: zone of inhibition around the disk


neg rxn: no zone of inhibition

Bile Esculin Agar

gram+ bact other than group D strep and entero are inh by the bile


indicator: ferric ammonium citrate


(dark brown color)


ferric ions to form a phenolic iron complex


pos rxn: blackening of the agar slant


neg rxn: no blackening

Bile Solubility Test

Principle: diff S. pneumoniae from alpha-hemolytic streptococci


lysis depends on the presence of intracellular autolytic enzymes


pos rxn: colony disintegrates


neg rxn: intact colonies

Cetrimide

principles: org to grow in the pre of cetrimide


pos: growth


neg: no growth

Citrate Utilization

principle: utilize sodium citrate as CO2 and inorganic ammonium salts as N


indicator: bromthymol blue from green to blue


pos: growth in thr medium w/ or w/o change in color of the indicator


neg: absence of growth