B1 W3 W, Defea 1

Front 1

What are integrins used for?

Back 1

1. allow communication between ECM and Cell and Cell-to-cell also..

Front 2

What are ways cells communicate with other cells?

Back 2

1. ECM
2. Contact dependant
3. paracrine
4. synaptic

Front 3

What are integrin ligands OUTSIDE the cell?

Back 3

1. fibronectins(CAM)
2. laminins
3. Ig
4. fibrinogen

Front 4

What are ligands INSIDE the cell

Back 4

1. tyrosine kinases
2. actin filaments

Front 5

What is anchorage dependant growth?

Back 5

its how cells proliferate, grow, and survive by attaching themselves to the substratum using integrins

Front 6

What signals can activate integrins to increase their affinity for ECM?

Back 6

1.Fibronectin molecules on Cell Adhesion Molecules
2. Cadherins

Front 7

What is a focal contact?

Back 7

when you assemble: actin filaments, integrins and Non receptor Tyrosine Kinases

Front 8

What are some NRTK

Back 8

SRC
FAK

Front 9

How are integrins involved in mitogenic signaling

Back 9

when fibronectin binds with the integrins they activate NRTK (SRC) and that leads to Ras--> Raf--->MEK--> ERK

Front 10

How do inegrins control growth

Back 10

You need integrin signal and a GF signal or else it does not activate for growth

Front 11

So do tumor cells have anchorage dependant growth

Back 11

No, they are anchorage INDEPENDANT growth
Why? they produce LESS fibronectin

Front 12

So how do integrins faciliate communication between the cytoskeleton and ECM

Back 12

1. outside in: fibronectin
2. Inside out: myosin

Front 13

So what are examples of Contact dependant signaling

Back 13

1. leukocyte extravasation w/ CAM
2. inhibition w/ Cadherins
3. Development w/delta notch
4. platelets

Front 14

So what is the Cell Adhesion Molecule Family (CAM)?

Back 14

1. Cadherins
2. IgCAM's
3. Selectin

Front 15

So which CAM is homophilic and is associated with actin filaments and adherence junctions?

Back 15

Cadherins
-Also a tumor supressor

Front 16

Which CAM mediate extravasation of blood cells and have hetero interactions?

Back 16

Selectins
They bind to Lectin found on the surface of cells

Front 17

Which CAM can bind to Ig and Integrins with either hetero or homo interactions

Back 17

IgCAM's

Front 18

So which CAM is important for Leukocyte Extravasation

Back 18

all three of them

Front 19

So all cells express Notch and Delta which bind together on adjacent cells, what cell will be turned on?

Back 19

If you inhibit your neighbors NOTCH you will win

Front 20

When comparing the amount of signal produced in synaptic and endocrine signaling which one produces its signal at higher concentrations?

Back 20

Sypnatic Signaling, because receptors have LOW affinity

Front 21

During neurosecretion what do the synaptic vessicles ride on?

Back 21

They go from using Kinesin on microtubles to myosin on actin filaments

Front 22

If you have a mutation affecting the Cadherins what can happen?

Back 22

Familial gastric cancer and colon cancer since you may loose contact growth inhibition

Front 23

What is Leukocyte Adhesion Deficiency Type 1?

Back 23

People have recurrent infections b/c leukocytes cannot extravasate out of the blood due to a mutation on the INTEGRIN