Applications Of Immunology: Elisa Stimulation Lab 15

Front 1

ELISA

Back 1

Enzyme Linked ImmunoSorbant Assay

Front 2

ELSA QUALITY

Back 2

because it feels you if an antibody is present

Front 3

ELSA QUANTITY

Back 3

amount of color change indicates how much of the antibody is present

Front 4

following the first buffer wash, what should be in the negative well, and in the positive well.

Back 4

neg= only the antigen at the bottom
pos= antibodies bound to antigen

Front 5

after the second buffer wash whats in the negative and positive test well

Back 5

neg= only the antigen at the bottom
pos= man made anti IgG antibodies, with conjugation enzymes, attached to antibodies attached to antigen

Front 6

why add chromogen?

Back 6

it is the substrate for the enzyme that is conjugated to the man made anti IgG antibodies. when the enzyme and substrate interact, a colored product produced. thus tells you whether the man made anti IgG antibodies are still present in the well or not

Front 7

when chromogen added what is absorbed to present color, what is absent to remain colorless

Back 7

the enzyme conjugated to man nade anti IgG antibodies

Front 8

why do we need controls
what makes the negative negaTive and why is this significant
and the positive

Back 8

to have known comparison
neg= no patient serum, if color occurs this indicates contamination
pos= contains HIV+ serum, no color solutions were not mixed properly

Front 9

positive

Back 9

chromogen color change (b)

Front 10

negative

Back 10

no color change (a)