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8 Cards in this Set
- Front
- Back
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What is a plasmid?
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A cicular, self replicating DNA.
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___ plamids, and __ chromosome
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many, one
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The multiple cloning site can be cut with ??
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enzymes(restriction endonuceleases)
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What is good about restiction endonuclease?
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Can cut plasmid to add in gene of interst.
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To make a ton of protein encoded on your gene of interst you want to use a plasmid vector with a very strong ____ upstream of the multiple cloning site.
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promoter.
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A very stong promoter will allow for ??
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A highly efficient RNA pol binding and therefore lots of mRNA.
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The enzyme that attaches the amino acid to the appropriate tRNA is ??
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Amino acyl tRNA synthase.
(proof reading) |
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What are the requirements for PCR?
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1)alot of primers
2)dNTPs 3)thermostable enzyme 4)Ions-buffers. |
