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122 Cards in this Set

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We are able to explore the incredible microscopic world that exists all around us due to the invention of a microscope. Who is credited for crafting the crude microscope and when did he do this?
The microscopic world was unknown to human beings until the 1670s when Anton van Leeuwenhoek crafted a crude microscope.
True or False? the combined weight of all microscopic organisms far exceeds the combined weight of all other living organisms on earth!
True.
True or False? The number of organisms from kingdom Monera that live in your body exceeds the number of cells that make up your body!
True.
Although bacteria are responsible for many illnesses that plague humanity (and other organisms), there are also many forms of bacteria that are beneficial to people. What are some of the things beneficial bacteria do for us?
a. bacteria in your gut synthesize B vitamins and vitamin K

b.bacteria help us make cheese

c.help make the earth hospitable to life

d. some are decomposers, performing the necessary job of recycling chemicals from dead organisms so that living organisms can use them
Since bacteria belong to kingdom Monera, they are prokaryotic. What does that mean?
Their cells have no distinct, membrane-bounded organelles.
When a pathogenic bacterium enters an organism, that organism has an infection-fighting mechanism which activates. So how does the pathogenic bacterium prevail and cause an infection?
The CAPSULE of the pathogenic bacterium can offer a significant amount of protection from the agents of this infection-fighting mechanism
What are the three basic bacterial shapes?
1. spherical bacteria are also known as coccus, the plural of which is cocci.

2. rod-shaped bacteria are known as bacillus, the plural of which are bacilli

3. helical bacteria are known as spirillum, the plural of which are spirilla
Since an electron microscope uses electron beams instead of light, the images that it produces are different from the actual item in what way?
The images that it produces do not have any color, so then colors you see in the images have been added after the image was acquired. (false-color images)
True or False? If the cell needs the chemicals, the plasma membrane will actually force the chemicals into the cell, whether they “want” to come in or not!
True
Fimbriae look like little legs or paddles and help the bacteria move about! True or False?
False. They don't support locomotion at all..... Either they grasp surfaces to help the bacteria adhere to them (as an aid to the sticky capsule), or they grasp other bacteria as a part of reproduction.
If a bacterium does not possess a flagellum, can it move at all?
Yes and no. The motion of substances in its surroundings might push it back and forth, but it is unable to move towards a goal.
The bacterium does not move by swishing the flagellum back and forth..... That is too simple. How does the flagellum move then?
The bacterium moves in a much more complex way, spinning like it has its own “outboard motor” that it uses to get from one point to another.
The symbol “μm” stands for _________ .
“micrometer,” a metric length measurement.
Thus, bacteria are really small. In fact, to give you an idea of exactly how small bacteria are, 10,000 of them would fit in the dot of this “i.” Now that's small!

True or False?
False. Actually, "only" about 1,000 would fit..... :o)
Bacteria have the ability to copy DNA in a mere 20 minutes. True or False?
True
Bacteria represent the “simplest” form of life on earth! True or False?
True. This tiny, complex organism is as simple as life gets.
Two different species of bacteria attempt to infect an organism. One bacterium succeeds, while the other is destroyed by the organism's infection-fighting mechanisms. What is most likely the major difference between these two bacteria?
The bacterium that succeeded most likely has a capsule while the other does not. In bacteria, the capsule helps to protect the organism from infection-fighting mechanisms.
A bacterium is poisoned by a substance that is allowed into the interior of the cell. What bacterial component did not do its job?
The plasma membrane did not do its job. Since it regulates what moves in and out of the cell, it should not have allowed the toxin to enter.
If a bacterium cannot move, what structure is it missing?
It is missing a flagellum. The fimbriae are not for movement; thus, if a bacterium has no flagellum, it cannot move.
From a cellular point of view, kingdom Monera is probably the most diverse kingdom in Creation. This is best illustrated by the diversity of eating habits among bacteria. Explain what is so diverse.
All life must have a means of converting energy from its surroundings into energy that will sustain the processes necessary for life. There are producers, consumers, and decomposers in kingdom Monera!
True or False? Most bacteria are consumers.
False! Most bacteria are decomposers.
Saprophytic bacteria. Why could these bacteria be celebrated on Earth Day?
Without the aid of these microscopic decomposers, many of the materials on earth that are necessary for life would be contained in the bodies of only a few generations of dead organisms. Sapropytic bacteria RECYCLE these materials back into creation so that living organisms can use them!
Not all heterotrophic bacteria are saprophytic. So what is the other option?
Some bacteria (the ones that cause disease, for example) are parasites.
In general, what are two different means by which autotrophic bacteria manufacture their own food?
photosynthesis or chemosynthesis
In green plants and most of the other photosynthetic organisms, the byproduct of photosynthesis is oxygen. The photosynthesis that occurs in some bacteria, however, does not have oxygen as a byproduct. Why?
This is because the chemicals that those bacteria use in photosynthesis are quite different from the chemicals used in the photosynthesis that is carried out by plants.
The main difference between chemosynthesis and photosynthesis is the source of energy. What are each of their energy sources?
photosynthesis = energy from sunlight

chemosynthesis = bacteria promote chemical reactions which release energy
Why do we breathe?
We breathe so that we get the oxygen we need in order to combine molecules with oxygen in a long, complicated chemical process that produces energy.
Why are pathogenic bacteria necessary to our planet?
Often, a group of organisms gets too large and could destroy the balance of nature. Typically, disease caused by pathogenic bacteria will kill off a large number of the organisms, making sure that the balance of nature remains intact.
What are bacteria that do not require oxygen called? Why are they important?
Anaerobic organisms, and they are important because If there were no decomposing bacteria in areas without oxygen (like swamp muck), the chemicals in dead organisms would never be recycled back into Creation. Because certain bacteria can be anaerobic, even though there is no oxygen down there, they will decompose the dead organism.
What was the purpose of Biosphere 2?
It was supposed to be a self-contained system for supporting life; a microcosm of life on earth. It contained a variety of animals and plants and was designed to be completely self-supporting.
Why did Biosphere 2 fail?
The scientists who designed Biosphere 2 did not take into account the incredibly valuable role that bacteria and other microscopic organisms play in life. Since these microorganisms were not present in the right amounts, Biosphere 2 could not maintain itself.
Where is Biosphere 2 and what purpose does it serve now?
Arizona, and it is now a hands-on, interactive science museum.
Can saprophytic bacteria be autotrophic?
No. Saprophytic means that it feeds on dead matter. Autotrophic organisms make their own food.
Can an aerobic bacterium be chemosynthetic?
Yes. Aerobic and anaerobic deal with how the organism converts its food into useful energy. Chemosynthetic deals with how the organism gets the food to begin with. Thus, the bacterium can make the food chemosynthetically and then convert it to useable energy aerobically.
True or False? Bacteria can only reproduce asexually.
True.
Although there are several ways in which this asexual reproduction can occur, the most popular form for bacteria to asexually reproduce is:
binary fission
How does asexual reproduction begin?
Reproduction begins when the DNA forms a loop attached to the plasma membrane.
True or False? The DNA copies itself.
True. Since reproduction forms a new living organism, new DNA must be formed.
What are the actual steps to reproduction?
1. DNA forms a loop attached to the plasma membrane

2.DNA then copies itself

3. the copy attaches to the plasma membrane close to the original

4. The cell wall and plasma membrane then elongate, separating the copy from the original.

5. The cell wall and plasma membrane begin to grow in between the two strands of DNA, eventually closing them off from one another.
How do mutations occur?
Mutations in asexual reproduction result when the DNA of the parent is not copied correctly.
If one bacterium could multiply into more than a billion bacteria in about 10 hours, why don't bacteria multiply out of control?
Even though bacteria can populate an area quickly, a limitation of resources will eventually slow the growth of the population until, when all resources run out, the population dies.
A population of bacteria grown from a single “starter” bacterium is rather fragile. When conditions are changed, the population dies quickly. Based on what you have just learned, develop a hypothesis for why this is the case.
Since asexual reproduction allows no variation in the DNA, an entire population of bacteria started from a single bacterium have all the same traits. If the environment changes, an organism might need new traits to survive. Since the whole population has essentially the same traits, there is no way to get the needed new traits, and the population dies. The variability that exists in sexual reproduction usually makes a population much more resistant to changes in the habitat.
A population of bacteria reaches a steady state and then, after several days, the population actually increases dramatically. What could cause such an event?
More resources (most likely food) were added to the habitat. The only way you can get population growth after the steady state would be due to an influx of new resources. In our cow example, maybe another cow died and fell on top of the first cow's body!
What are the three ways genetic recombination can occur?
conjugation,
transformation,
or transduction
Why is conjugation different from sexual reproduction? After all, they are engaged in a temporary union of two organisms for the purpose of DNA transfer.....
When two bacteria conjugate, no offspring is formed. Instead genetic information is transferred from a donor bacterium to a recipient.

Also, in most cases of sexual reproduction, each parent contributes DNA, and the offspring's DNA is a blend of parents' DNA. In conjugation among bacteria only one organism donates DNA.
Why does this strange process called conjugation occur?
Well, in a population of bacteria, there may be a few individuals with traits that are different from the traits of the others. If these traits are desirable, it would benefit the population if they were passed on to its other members.
Which bacterium is the one that initiates conjugation? The donor bacterium or the recipient bacterium?
Research indicates that the donor bacterium is the one that initiates conjugation.
DNA is made up of two strands that are joined in the shape of a double helix. So when a bacterium donates one strand, how does it get the second DNA strand it needs?
It turns out that due to the chemical nature of DNA, if an organism has one strand, it can construct the other strand using the first as a template. Thus, as the strand is being transferred, the donor bacterium begins making a second strand for the plasmid to replace the strand that it is donating.
A population of bacteria are living in a lake. Due to volcanic activity nearby, the lake's temperature begins to increase. In the population, there are some bacteria that are resistant to low temperatures (call them type A) and another type that are resistant to high temperature (call them type B). Which type will be the donor and which the recipient as the population begins to conjugate?
Type B will be the donors and Type A will be the recipients. Since the bacteria will need to survive in high temperatures, they need resistance to high temperatures. That's what will be donated.
Other than conjugation, what is another way for a bacterium to obtain a plasmid?
Transformation! In this process, a dead bacterium's DNA seems to break into small pieces, and a living bacterium might “sense” that one or more of those pieces contains a trait or traits that it could use. The living bacterium then absorbs what it needs, incorporating the new genetic information as a plasmid.
True or False? A virus can pick up DNA from a bacterium during infection and that DNA can be inserted into another bacterium in a subsequent infection.
True. This is called Transduction.
Boiling water kills bacteria making the water safe to drink. True or False?
False. Although boiling water will kill the bacteria, they can actually survive the high temperature for some period of time by producing an endospore.
Once an endospore forms, the bacterium itself might die, but as long as the endospore survives, the bacterium can reform.
If bacteria do not have to be a part of a colony to survive, why would they bother being part of a colony at all?
Since there is strength in numbers, the bacteria's ability to survive is usually enhanced when they form a colony.
What are the most common bacterial colonies made up of, shape-wise?
The most common bacterial colonies are made up of either cocci or bacilli bacteria.
The shape of the bacteria is indicated in the name. Explain....
If the bacteria are spherical, the name ends in “coccus.” If they are rod-shaped, the name ends in “bacillus.”
Some colonies will secrete a capsule-like substance. Why do they do this?
This substance gives the bacterial colony a slimy feel and holds the colony together. It surrounds and protects the entire colony. (found in places like stagnant ponds)
How do other colonies work together to capture and eat prey?
A Myxococcus xanthus colony, for example, moves as a unit in search of prey, typically other bacterial colonies. When they find their prey, they engulf it and, as a group, secrete a substance that digests it. The entire colony then feeds on the nutrients.
If a streptococcus bacterium is separated from its colony, how does it know which other colony to join?
The DNA seems to tell them what colony they should form or join. It won't join up with just any other colony. It will continue to live independently until it can find another streptococcus colony. If it never finds one, it will live out its life as an independent bacterium.
A bacterial colony is called staphylobacillus. What shape do the bacteria in the colony possess: spherical, rod-shaped, or helical?
They are rod-shaped, since “bacillus” means rod-shaped.
When you say that a jar is a "culture"..... what do you mean?
Jars are often called cultures when microorganisms are being cultured inside them to produce more microorganisms.
Who developed a way to make bacteria show up better under a microscope? When? How?
In the late 1800s, a Danish physician named Hans Christian Gram developed several different types of stains, now referred to as Gram stains.
Which bacteria look red after the Gram stain?
Gram-negative bacteria are the ones that look red after the Gram stain.
Which bacteria look blue after the Gram stain?
Gram-positive bacteria are the ones that look blue following the Gram stain
What causes the different colors?
The difference in reaction to a Gram stain is caused by differences in the cell walls of these bacteria. Bacteria which are Gram-positive have cell walls which retain the Gram stain so that it stays in the cell.
What is the first way that we separate the organisms in kingdom Monera?
The first way we separate the organisms in kingdom Monera, then, is by their cell walls.
Gram-negative bacteria belongs to which phylum?
Gram-negative belongs to phylum Gracilicutes.
Gram-positive bacterium belongs to which phylum?
Firmicutes
What do you do with the bacteria that have no cell walls at all? How do you classify them?
These bacteria are put into phylum Tenericutes.
Some bacteria possess cell walls, but the compounds which form these walls are rather different from the compounds that form the cell walls of Gram-positive and Gram-negative bacteria. What phylum do they belong to?
Mendosicutes
A bacterium has no cell wall. To what phylum does it belong?
Tenericutes is the phylum for bacteria without a cell wall.
A bacterium is classified as Gram-positive. To what phylum does it belong?
Firmicutes contains all Gram-positive bacteria.
A bacterium appears red after a Gram stain. To what phylum does it belong?
Gracilicutes contains all Gram-negative bacteria. Since the bacterium appears red after the Gram stain, it is Gram-negative.
A bacterium has a cell wall that is different from both Gram-positive and Gram-negative bacteria. To what phylum does it belong?
Mendosicutes contains all bacteria with exotic cell walls.
What are the four phylums in Kingdom Monera?
1. Gracilicutes
2. Firmicutes
3. Tenericutes
4. Mendosicutes
Phylum Gracilicutes (Gram-negative bacteria) has three classes. How are they separated from one another?
We separate members of this phylum based on how they obtain their food.
What are the three classes of the phylum Gracilicutes?
1. Scotobacteria
2. Anoxyphotobacteria
3. Oxyphotobacteria
Many pathogenic bacteria belong in which class of the phylum Gracilicutes?
Scotobacteria
Which class is made up of photosynthetic bacteria that produce oxygen? (phylum Gracilicutes)
Oxyphotobacteria
If a bacteria lives in the sediments of lakes or rivers, which class does it belong to? (phylum Gracilicutes)
Anoxyphotobacteria
You see blue-green algae (known as cyanobacteria)floating on the top of a stagnant pond.
What class would you classify it as? (phylum Gracilicutes)
Oxyphotobacteria because it is made up of photosynthetic bacteria that produce oxygen.
How many classes are in the phylum Firmicutes, and what are they?
In phylum Firmicutes, there are only two classes: Firmibacteria and Thallobacteria
How are the classes in Firmicutes divided?
They are separated based on their shape!
Class Firmibacteria contains which shape/shapes?
Class Firmibacteria contains cocci and bacilli bacteria.
Thallobacteria contains which shape/shapes?
Class Thallobacteria is made up of any other shape...
Why do the last two phyla ~Tenericutes and Mendosicutes~ have only one class each?
This is because there are few bacteria that fall into these phyla.
The class Mollicutes..... which phylum does it belong to?
Phylum Tenericutes has the class Mollicutes.
Mendosicutes contains the class Archaebacteria. Why could this class be considered special?
Archaebacteria holds all of the bacteria with exotic cell walls. These exotic cell walls allow members of class Archaebacteria to live in rather odd locations, such as deep-ocean hydrothermal vents or incredibly brackish seas like the Great Salt Lake. Many places that are uninhabitable to other organisms are populated with members of this class.
What do the three-domain and six-kingdom classification systems have in common?
They split up kingdom Monera.
What does Class Archaebacteria use to convert food into energy if they don't have oxygen?
They use hydrogen and carbon dioxide.
True or False? Cyanobacteria and blue-green algae are the same thing.....
True.
True or False? Blue-green algae isn't really algae.
True. These organisms looked like algae, so they were at first incorrectly named as such. Today, we recognize them as colonies of Gram-negative prokaryotic cells and thus classify them in kingdom Monera
Clostridium botulinum is responsible for what condition?
It can be a source of food poisoning. The endospores that it forms are quite heat resistant, so when food has not been properly cooked, this bacterium can survive. It produces a toxic chemical that causes the disease known as botulism
Salmonella typhimurium and S. enteriditis are both forms of the bacteria Salmonella. True or False?
True. Remember, once you have written the genus name you can abbreviate it afterward. So the "S." stands for Salmonella.
What is the only way to get rid of Salmonella bacteria?
The only way to make sure you get rid of them is by cooking the meat or eggs thoroughly enough to kill the bacteria and their endospores.
True or False? Salmonella can be very serious... even causing death!
True.
Escherichia coli is found in your gut. Also known as E. coli, why would you WANT this to be in your gut?
1. One of the byproducts of E. coli's metabolism is Vitamin K, a substance your body needs

2. It also secretes a chemical that helps your body digest fat

3. Its activities in your gut actually keep food-borne pathogenic bacteria from colonizing there.
So if E. coli has such beneficial aspects to it, why does it give you severe diarrhea?
Actually, the E. coli bacterium found in your gut is nonpathogenic, but there are pathogenic forms of E. coli.
How can there be both pathogenic and nonpathogenic E. coli? They are all the same species!?
When bacteria develop in different habitats, they can develop different traits.

When two organisms from the same species have markedly different traits, we say that they are from two different strains.
True or False? There are only three strains of a bacterial species.
False.... there can be many strains of a bacterial species
What conditions must be present in order for populations of bacteria to grow?
1. Moisture
2. Moderate temperature
3. Nutrition
4. Darkness (some exceptions)
5. The proper amount of oxygen
What is the "ideal" time for bacterial growth, and approximately what temperatures are ideal?
27 °C - 38 °C (80 °F - 100 °F). This makes summer the most ideal time for bacterial growth.
True or False? Bacterial endospores are even in the dust particles that fly through the air.....
Yes, this is true.
How can you prevent bacterial infections from your food?
1. expose the food to extreme heat or radiation and then seal it in a container before exposing it to fresh air

2. dehydrate the food

3. freeze the food

4. refrigerating it will SLOW the growth of bacteria
Louis Pasteur developed a process called pasteurization, which he originally used for what purpose? What use is it to us now?
He originally used to keep wine from souring, but it is used all of the time now in milk production.
Why should milk be covered and refrigerated at all times?
Exposure to air will allow bacteria back into the milk.
Draw the figure pictured in your study guide and label the individual components.
draw figure
Describe the functions of each:

plasma membrane
flagellum
capsule
DNA
cytoplasm
cell wall
fimbria
ribosome
a. Plasma membrane: To negotiate what materials pass into and out of the cell

b. Flagellum: To move the bacterium from place to place

c. Capsule: To adhere to surfaces as well as to ward off infection-fighting agents

d. DNA: To store the information needed to make an organism a living thing

e. Cytoplasm: To hold the DNA and ribosomes in place

f. Cell wall: To keep the interior of the cell together and to hold the cell’s shape

g. Fimbria: To grasp onto surfaces or another bacterium during conjugation

h. Ribosome: To make proteins
What is the most popular means by which bacteria obtain food?
Most bacteria are heterotrophic decomposers.
If a bacterium is parasitic, is it heterotrophic or autotrophic?
Parasites, by definition, feed on something produced by a host. They therefore cannot make their
own food. This makes them heterotrophic.
List the basic steps in asexual reproduction among bacteria
At first, the DNA loop attaches to a point on the plasma membrane. After that, the DNA is copied,
and the copy is attached to a point on the plasma membrane near the original. Then, the cell wall
elongates, which separates the two loops of DNA. Once they are sufficiently separated, new cell wall
and plasma membrane material grow, closing the two loops off from each other. Eventually, the cell
wall and plasma membrane pinch down, forming two cells where there was only one before.
A sample of food is dehydrated, which kills all of the bacteria. However, in a few days, bacteriafree
water is added to the food in a bacteria-free environment. Nevertheless, microscopic
investigations indicate that bacteria are in the food. How did the bacteria get there?
The bacteria that are there were in the food as endospores. The bacteria that were in the food before
it was dehydrated formed endospores to survive a little while without water. When water was added to
the food, the conditions were once again favorable for bacteria, so the cells burst from the endospores.
. What kind of growth does a population of bacteria experience when just a few of them are placed in
an environment rich with resources?
If the environment is rich with resources and there are only a few bacteria, the population will
experience exponential growth, because there is nothing to limit their reproduction.
A population of bacteria experiences logistic growth. What can you say about the resources of their
environment?
Since the growth is logistic, the resources are limited but do no run out. If the resources ran out, the
population would experience a decline, and that is not a part of logistic growth.
. Even though genetic recombination among bacteria does not result in offspring, it can significantly
affect the growth of the population. Why?
Genetic recombination can pass a trait from one bacterium to another. If that trait allows the
recipient to survive conditions that it otherwise wouldn’t, the population is affected, because the
recipient continues to live and reproduce asexually
What are the technical names of the three common bacterial shapes?
Coccus - Spherical
Bacillus - Rod-shaped
Spirillum - Helical
A bacterium is heterotrophic and Gram-negative. To what phylum and class does it belong?
. If it is Gram-negative, it is in phylum Gracilicutes. In this phylum, two classes contain
photosynthetic bacteria, which are autotrophs. Since this bacterium is heterotrophic, it must belong to
the only other class, class Scotobacteria.
A spirillum bacterium is Gram-positive. To what phylum and class does it belong?
Gram-positive means phylum Firmicutes. Since it is spirillum-shaped, it is neither coccus nor
bacillus. Thus, it is in class Thallobacteria.
A bacterium has no cell wall. To what phylum and class does it belong?
. Bacteria with no cell walls belong to the phylum Tenericutes, which has only one class, class
Mollicutes
A bacterium lives in boiling-hot water. To what phylum and class does it most likely belong?
This bacterium most likely is in phylum Mendosicutes and class Archaebacteria, because bacteria
with exotic cell walls can survive conditions that other organisms cannot.
In the six-kingdom classification system, what replaces kingdom Monera?
In the six-kingdom system, kingdom Monera is split into kingdom Archaebacteria and kingdom
Eubacteria.
. What conditions are ideal for most bacteria to grow and reproduce?
To grow and reproduce, ideal conditions for most bacteria include: Moisture, moderate
temperatures, nutrition, darkness, and the proper amount of oxygen.
What methods exist for reducing the chance of bacterial contamination of food?
To reduce the chance of bacterial infection you can:

a) Heat the food so that most bacteria die and then seal it away from fresh air.
b) Dehydrate the food.
c) Freeze the food.
d) Pasteurize the food.
e) Keep it in the refrigerator.