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46 Cards in this Set
- Front
- Back
• Water is ___ CO2 is ___ in photosynthesis
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oxidized
reduced |
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• Goal of light reactions
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use sunlight to make ATP and NADPH
convert solar energy to chemical energy |
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• Goal of Calvin Cycles
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make sugars from ATP and NADPH
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• Calvin cycle must spin __ times to make __ glucose
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6
1 |
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• Calvin cycle must spin __ times to make __ PGAL (2 PGAL=1 glucose)
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3
1 |
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• Autotrophs
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sustain themselves without eating anything derived from other organisms- produce organic compounds from inhaling CO2- ultimate sources of organic compounds for non-autotrophic organisms
o Aka producers o Plants are autotrophs |
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• Photoautotrophs
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specifically plants
o Organisms that use light as a source of energy to synthesize organic substances |
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• Heterotrophs
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obtain organic material by eating other things
o Aka consumers o Can’t make their own food |
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• Chlorophyll
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pigment that gives plants a green color
o Located in chloroplasts o Found in mesophyll of chloroplast (tissue in the interior of the leaf) |
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• Stomata
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microscopic pores in leaves- facilitates exchange of gasses like O2 and CO2
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• Stroma
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dense fluid within the chloroplast
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• Thylakoids
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membranous sacs within a chloroplast- separates stroma from thylakoid space→ holds chlorophyll in Thylakoid membrane
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• Granum
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-→ a stack of thylakoid membranes
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Photosynthesis equation
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• 6CO2 + 6 H2O + Light→ C6H12O6 + 6O2
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• Chloroplasts split ___ into ____
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water
hydrogen and oxygen |
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• Light reaction
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o Convert solar energy to chemical energy
o Light absorbed by chlorophyll drives a transfer of electrons and hydrogen from water to NADP+ o Water is split o Light reaction gives off O2 as a by product o Electron acceptor in light reactions= NADP+ • NADP+ becomes NADPH when light energy adds 2 electrons and a H+ ion o Light reaction generates ATP through chemiosmosis (photophosphorylation) o Light energy gets converted to 2 things→ ATP or NADPH |
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• Calvin cycle
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o Takes NADPH CO2 and ATP and makes sugar
o Carbon fixation→ initial incorporation of carbon into organic compounds o Calvin cycle begins by incorporating CO2 from the air into organic molecules already present in the chloroplast o CC reduces the fixed carbon to carbohydrate by adding electrons (through NADPH) o CC does not directly require light • Rely on NADPH and ATP from the light reactions |
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• Light reaction happens in
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thylakoids
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• Calvin cycle happens in
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stroma
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o Wavelength
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distance between crests of electromagnetic waves
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o Electromagnetic spectrum
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GXUVIMR
Gamma- short wavelength high energy Radio- long wavelength low energy |
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visible light spectrum
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ROYGBIV
Red- long wavelength low energy Violet- short wavelength high energy |
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photons
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o Light behaves as though it consists of discrete particles called photons
• Each photon has a fixed quantity of energy |
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• When light hits matter it is either...
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reflected transmitted or absorbed
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• Pigments
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substances that absorb visible light
o The color you see is the color reflected back- the color it doesn’t absorb o If it absorbs all wavelengths the color is black |
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• Spectrophotometer
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measures the ability of a pigment to absorb various wavelengths of light
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• Chlorophyll a
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absorbs violet and blue, reflects blue and green
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• Chlorophyll b
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absorbs violet and blue, reflects yellow and green
o Almost the same as chlorophyll A but slightly structurally different |
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• Carotenoids
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reflect yellow orange and red - absorb violet blue and green
o In the fall, plants stop making chlorophyll and all you have left are the carotenoids |
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o Photoprotection
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carotenoids absorb and dissipate excessive light energy that would otherwise damage chlorophyll or interact with oxygen
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Excitation of chlorophyll by light
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• Ground state→ electron is in its normal orbital
• Absorption of a photon boosts an electron to an orbital of higher energy (excited state) o The photons absorbed are those whose energy is exactly equal to the energy difference between the ground and excited state • When the electrons drop back down from the excited to the ground state they release energy (in the form of heat and sometimes light) |
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o Fluorescence
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the afterglow from when after the electrons fall back down and release heat/light
o Isolated Chlorophyll will fluoresce and give off heat because it's not in a chloroplast and there's no electron acceptor to harness the energy |
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• Photosytem
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reaction center associated with light harvesting complexes
o Composed of a reaction center surrounded by a number of light harvesting complexes |
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• Light harvesting complex
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consists of pigment molecules (chlorophyll a or b or carotenoids)
o Number and variety of pigment molecules enable a photosystem to harvest light over a larger surface and larger portion of the spectrum |
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• Reaction center
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protein complex that includes two chlorophyll a molecules and a primary electron acceptor
o Light hits Chlorophyll a and it transfers an electron to the primary electron acceptor (first step of light reactions) • Electron jumps up and gets caught by the primary electron acceptor |
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• 2 kinds of photosystems-→
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Photosystem II and photosystem I
o both have a reaction center, primary electron acceptor, chlorophyll a molecules, 2 comes before 1 |
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• Noncyclic electron flow
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o The usual electron route through the light reactions
o Makes NADPH and ATP o Pushes electrons from water (low state of potential energy) to NADPH (high state of potential energy) |
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• Cyclic electron flow
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second path of electron flow through the light reactions
o Uses PS I and not PS II o Only makes ATP (no NADPH) o Since the calvin cycle consumes more ATP than NADPH cyclic electron flow helps power the calvin o Concentration of NADPH in the chloroplast could help regulate which pathway (cyclic or non cyclic) the electrons travel through |
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differences between mitochondria oxidative phosphorylation and chloroplast photophosphorylation
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• Oxidative phosphorylation→ mitochondria
o Electrons are extracted from organic molecules o Transfers chemical energy to ATP o Inner membrane pumps protons from matrix to intermembrane space • Photophosphorylation→ chloroplasts o Use light to drive electrons down the chain o Transfers light energy to ATP and NADPH o Thylakoid membrane pumps protons from stroma to thylakoid space o Thylakoid membrane makes ATP o ATP forms in stroma |
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• Calvin cycle is ___
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anabolic (builds sugar from smaller low energy molecules- consumes energy)
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photorespiration
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o Plants perform photorespiration when CO2 levels are low (when the stomata are closed when it's dry outside)
occurs in light, consumes O2, produces CO2 o Consumes rather than generates ATP o Doesn’t produce sugar o Decreases photosynthetic output o Very counterproductive process |
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transpiration
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o Water moves through the stomata
• When it’s dry outside the plant loses water (transpiration) • Transpiration→ evaporation of water from leaves |
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• C3 plants
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o Rice, wheat, soybeans
o Initial fixation of carbon occurs via Rubisco o First organic product of carbon fixation is 3 phosphoglycerate o Rubisco binds to either O2 or CO2 o When Rubisco binds to O2 the plant photorespires |
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• C4 plants
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o Sugarcane, corn, grasses
o Two distinct types of photosynthetic cells • Bundle sheath cells • Arranged into tightly packed sheaths around the veins of the leaf • Calvin cycle happens here • Mesophyll cells • Carbon fixation happens here o Pep carboxylase→ fixes the carbon • Higher affinity for CO2 and no affinity for O2 • More efficient than rubisco o Mesophyll cells in C4 plants pump CO2 into the bundle sheath • Keeps CO2 concentrations high so the rubisco can bind to CO2 and not O2 o C4 photosynthesis minimizes photorespiration and enhances sugar production • Advantageous in hot dry regions o Calvin cycle occurs in bundle sheath and carbon fixation occurs in mesophyll |
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• CAM plants
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o Cactus, pineapples, jade plants
o Open stomata at night when it’s not as hot out • Helps them to conserve water o CAM= crassulacean acid metabolism o Preform the light reactions during the day and the calvin cycle at night o Calvin cycle and carbon fixation occur in the same cell |
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• Plants make more organic material each day than they need so_____
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they store extra sugar in the form of starch
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