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46 Cards in this Set

  • Front
  • Back
• Water is ___ CO2 is ___ in photosynthesis
oxidized

reduced
• Goal of light reactions
use sunlight to make ATP and NADPH

convert solar energy to chemical energy
• Goal of Calvin Cycles
make sugars from ATP and NADPH
• Calvin cycle must spin __ times to make __ glucose
6

1
• Calvin cycle must spin __ times to make __ PGAL (2 PGAL=1 glucose)
3

1
• Autotrophs
sustain themselves without eating anything derived from other organisms- produce organic compounds from inhaling CO2- ultimate sources of organic compounds for non-autotrophic organisms
o Aka producers
o Plants are autotrophs
• Photoautotrophs
specifically plants
o Organisms that use light as a source of energy to synthesize organic substances
• Heterotrophs
obtain organic material by eating other things
o Aka consumers
o Can’t make their own food
• Chlorophyll
pigment that gives plants a green color
o Located in chloroplasts
o Found in mesophyll of chloroplast (tissue in the interior of the leaf)
• Stomata
microscopic pores in leaves- facilitates exchange of gasses like O2 and CO2
• Stroma
dense fluid within the chloroplast
• Thylakoids
membranous sacs within a chloroplast- separates stroma from thylakoid space→ holds chlorophyll in Thylakoid membrane
• Granum
-→ a stack of thylakoid membranes
Photosynthesis equation
• 6CO2 + 6 H2O + Light→ C6H12O6 + 6O2
• Chloroplasts split ___ into ____
water

hydrogen and oxygen
• Light reaction
o Convert solar energy to chemical energy
o Light absorbed by chlorophyll drives a transfer of electrons and hydrogen from water to NADP+
o Water is split
o Light reaction gives off O2 as a by product
o Electron acceptor in light reactions= NADP+
• NADP+ becomes NADPH when light energy adds 2 electrons and a H+ ion
o Light reaction generates ATP through chemiosmosis (photophosphorylation)
o Light energy gets converted to 2 things→ ATP or NADPH
• Calvin cycle
o Takes NADPH CO2 and ATP and makes sugar
o Carbon fixation→ initial incorporation of carbon into organic compounds
o Calvin cycle begins by incorporating CO2 from the air into organic molecules already present in the chloroplast
o CC reduces the fixed carbon to carbohydrate by adding electrons (through NADPH)
o CC does not directly require light
• Rely on NADPH and ATP from the light reactions
• Light reaction happens in
thylakoids
• Calvin cycle happens in
stroma
o Wavelength
distance between crests of electromagnetic waves
o Electromagnetic spectrum
GXUVIMR

Gamma- short wavelength high energy

Radio- long wavelength low energy
visible light spectrum
ROYGBIV

Red- long wavelength low energy

Violet- short wavelength high energy
photons
o Light behaves as though it consists of discrete particles called photons
• Each photon has a fixed quantity of energy
• When light hits matter it is either...
reflected transmitted or absorbed
• Pigments
substances that absorb visible light
o The color you see is the color reflected back- the color it doesn’t absorb
o If it absorbs all wavelengths the color is black
• Spectrophotometer
measures the ability of a pigment to absorb various wavelengths of light
• Chlorophyll a
absorbs violet and blue, reflects blue and green
• Chlorophyll b
absorbs violet and blue, reflects yellow and green
o Almost the same as chlorophyll A but slightly structurally different
• Carotenoids
reflect yellow orange and red - absorb violet blue and green

o In the fall, plants stop making chlorophyll and all you have left are the carotenoids
o Photoprotection
carotenoids absorb and dissipate excessive light energy that would otherwise damage chlorophyll or interact with oxygen
Excitation of chlorophyll by light
• Ground state→ electron is in its normal orbital
• Absorption of a photon boosts an electron to an orbital of higher energy (excited state)
o The photons absorbed are those whose energy is exactly equal to the energy difference between the ground and excited state
• When the electrons drop back down from the excited to the ground state they release energy (in the form of heat and sometimes light)
o Fluorescence
the afterglow from when after the electrons fall back down and release heat/light

o Isolated Chlorophyll will fluoresce and give off heat because it's not in a chloroplast and there's no electron acceptor to harness the energy
• Photosytem
reaction center associated with light harvesting complexes
o Composed of a reaction center surrounded by a number of light harvesting complexes
• Light harvesting complex
consists of pigment molecules (chlorophyll a or b or carotenoids)
o Number and variety of pigment molecules enable a photosystem to harvest light over a larger surface and larger portion of the spectrum
• Reaction center
protein complex that includes two chlorophyll a molecules and a primary electron acceptor
o Light hits Chlorophyll a and it transfers an electron to the primary electron acceptor (first step of light reactions)
• Electron jumps up and gets caught by the primary electron acceptor
• 2 kinds of photosystems-→
Photosystem II and photosystem I
o both have a reaction center, primary electron acceptor, chlorophyll a molecules,

2 comes before 1
• Noncyclic electron flow
o The usual electron route through the light reactions
o Makes NADPH and ATP
o Pushes electrons from water (low state of potential energy) to NADPH (high state of potential energy)
• Cyclic electron flow
second path of electron flow through the light reactions
o Uses PS I and not PS II
o Only makes ATP (no NADPH)
o Since the calvin cycle consumes more ATP than NADPH cyclic electron flow helps power the calvin
o Concentration of NADPH in the chloroplast could help regulate which pathway (cyclic or non cyclic) the electrons travel through
differences between mitochondria oxidative phosphorylation and chloroplast photophosphorylation
• Oxidative phosphorylation→ mitochondria
o Electrons are extracted from organic molecules
o Transfers chemical energy to ATP
o Inner membrane pumps protons from matrix to intermembrane space


• Photophosphorylation→ chloroplasts
o Use light to drive electrons down the chain
o Transfers light energy to ATP and NADPH
o Thylakoid membrane pumps protons from stroma to thylakoid space
o Thylakoid membrane makes ATP
o ATP forms in stroma
• Calvin cycle is ___
anabolic (builds sugar from smaller low energy molecules- consumes energy)
photorespiration
o Plants perform photorespiration when CO2 levels are low (when the stomata are closed when it's dry outside)

occurs in light, consumes O2, produces CO2
o Consumes rather than generates ATP
o Doesn’t produce sugar
o Decreases photosynthetic output
o Very counterproductive process
transpiration
o Water moves through the stomata
• When it’s dry outside the plant loses water (transpiration)
• Transpiration→ evaporation of water from leaves
• C3 plants
o Rice, wheat, soybeans
o Initial fixation of carbon occurs via Rubisco
o First organic product of carbon fixation is 3 phosphoglycerate
o Rubisco binds to either O2 or CO2
o When Rubisco binds to O2 the plant photorespires
• C4 plants
o Sugarcane, corn, grasses
o Two distinct types of photosynthetic cells
• Bundle sheath cells
• Arranged into tightly packed sheaths around the veins of the leaf
• Calvin cycle happens here
• Mesophyll cells
• Carbon fixation happens here
o Pep carboxylase→ fixes the carbon
• Higher affinity for CO2 and no affinity for O2
• More efficient than rubisco
o Mesophyll cells in C4 plants pump CO2 into the bundle sheath
• Keeps CO2 concentrations high so the rubisco can bind to CO2 and not O2
o C4 photosynthesis minimizes photorespiration and enhances sugar production
• Advantageous in hot dry regions
o Calvin cycle occurs in bundle sheath and carbon fixation occurs in mesophyll
• CAM plants
o Cactus, pineapples, jade plants
o Open stomata at night when it’s not as hot out
• Helps them to conserve water
o CAM= crassulacean acid metabolism
o Preform the light reactions during the day and the calvin cycle at night
o Calvin cycle and carbon fixation occur in the same cell
• Plants make more organic material each day than they need so_____
they store extra sugar in the form of starch