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12 Cards in this Set
- Front
- Back
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Define: Histology
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Study of cells, tissues, organs at the microscopic level
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What are the limitations for the light microscope?
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0.25 um to 1 mm
-see big things, aorta vessels nuclei and nuceloi -mitochondria |
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What are the limitations for the electron microscope?
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We can see 0.2 nm to 0.25 um
-We can see cytoskeletal elements, mitochondria |
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What are the basic steps towards processing tissue?
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1) Fix it
2) Dehydrate tissue 3) Embed tissue 4) Section tissue 5) Stain Tissue |
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How do we fix the tissue?
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We cross link the protein and tissue together, to maintain the tissue architecture and we use aldehydes and formaldehydes to do this
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How do we dehydrate the tissue?
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We use ethanol
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How do we embed the tissue?
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We embed into hard material that can be cut into thin slices
-For light micro, we use wax, parafilm -For electron, we use plastic |
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How do we section the tissue?
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L.M- we cut think sections 5 to 10 um thick
E.M- cut 25 to 100 nm thick |
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How do we stain the tissue?
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L.M- Histochemical, seize reactive groups on molecules such as charge (sugars) and this is not specific
E.M- use immunostaining, for specific antigens, (specific) -Heavy metals for transmission EM gold and metal coating for scanning |
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What are tissues
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group of similar cells and surrounding extracellular matrix and extraceullar fluid (also known as intercellular)
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4 major tissue types
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epithelia, connective tissue, muscle, nervous tissue
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what are organs,
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they are made up of groups of tissues that act together to carry out specific bodily functions
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