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9 Cards in this Set
- Front
- Back
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Max. resolution of optical microscope |
0.2 micrometres Can barely see mitochondria; can see nucleus |
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Max. resolution of electronmicroscope |
0.0002 micrometres |
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Max. useful magnification of optical microscope |
x 1500 |
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Max. useful magnification of electron microscope |
x 1 500 000 |
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Limitations of Electron Microscope |
Specimen vacuum; can't be living Complex staining process; may leave artefacts hard to distinguish from actual specimen Image not in colour Specimen must be v. thin (except for scanning EM) |
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Conditions for homogenisation |
Ice cold soln; reduce activity of enzymes which may break down organelles Isotonic soln. Buffer soln. |
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Term for the lightest organelles at top of tube in ultracentrifugation |
Supernatant |
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Stages of cell fractionation |
Homogenisation Filtration Ultracentrifugation |
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Term for heavy organelles at bottom |
Pellet |
