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133 Cards in this Set

  • Front
  • Back

Locomotory apparatus of Amoeba spp

Pseudopodia


Flagella


Cilia

Only pathogenic amoeba

E. hystolitica

lowest form of animals

Protozoans

Vegetative stage


Feeding stage


Motile stage

Trophozoite

Resistant to environment


Infective Stage


Non-motile

Cyst

Morphologically similar with E. hysto

E. disparr


E. moschkovski


E. bangladeshi

Causes Amoebic Liver Abscess

E. hystolitica

Amoeba with ingested RBC

E. hysto

Small race E. hystolitica

E. hartmanii

Amoeba with large glycogen vacuole

Iodamoeba butschlii

Extraintestinal amoeba with ingested WBC in cytoplasm

E. gingivalis

Shape of Pseudopodia:



1. E hysto



2. E coli

1. Finger-like



2. Blunt

Release of Pseudopodia:



1. E. hysto



2. E. coli

1. one at a time/ explosive



2. several at a time/ slow manner

Stored food of parasites

Chromatoidal bars

Charcot Leyden Crystals in:


1. Amoebic dysentery


2. Bacillary Dysentery

1. (+)


2. Neg

Pus cells/ Neutrophil:



1. Amoebic dyse.



2. Bacillary dyse.

1. Few



2. Numerous

Anchovy- like disease

Hepatic amoebiasis

Virulence Factor of E hysto

Gal Nac Lectin : Adherence


Amoeba Pores : Pore former


Cysteine Proteinase : Destroys tx

Lab Dx that detects live motile troph

DFS- NSS

Wet stain for cyst (with glycogen and starch)

Lugol's Iodine

Concentration Techniques of amoeba cyst

Merthiolate Iodine Formaaldehyde CT



FECT

Only Parax w/o proper classification

Blastocystis hominis

Organisms without proper classification

Stramenophiles

Not merely a commensal but regarded as a potential pathogen

B. hominis

With large vacuole, nucleus are pushed to the side.

B. hominis

Culture of B. hominis

Boeck/ Dorbohlav's/ Nelson and Jones media

Brain eating amoeba

N. fowleri

Pathox of N. fowleri

Primary Amoebic MeningoEncephalitis

Causes Granulomatous Amoebic Meningoencephalitis

Acanthamoeba

PYGC agar

Acanthamoeba

Free Living amoeba with (+) of Spines projection

Acanthamoeba

Free living amoeba with 3 forms

N. fowleri

Most pathogenic intestinal flagellates

G. lamblia

source of energy of flagellates

Parabasal body

Support: Rod like structure of flagellates

Axostyle

Rib-like for support

Costa

Monkey- faced parasite

G.lamblia

G. lamblia trophozoite

4 pairs of flagella


2 ventral sucking disc

Covered with Variant-specific surface protein

G.lamblia

Cyst of G lamblia

Ovoid


Quadrinucleated

"Daycare outbreak"

G. lamblia

Lab Dx of G lamblia

Beale's string test/ Entero test

Safety Pin appearance flagellate

C. mesnilii

Corksrew motility

C. mesnilii

Niple shaped cyst


Lemon shaped cyst

C. mesnilii

Uninucleated cyst with distinct anterior protruberance

C. mesnilii cyst

Commensal flagellate but can cause diarrhea

D. fragilis

Habitat of D. fragilis

Mucosal crypts of the colon

Flagellate without a cyst form

D. fragilis

Morphology of D. fragilis

3-18 mm, hyaline broad and leaf like pseudopodia, binucleated

Jerky tumbling motility

T.vaginalis

Has prominent siderophil granules

T. vaginalis

Trichomonas that resides in the mouth and has the longest Undulating membrane

T. tenax

Pear shaped


4 anterior flagella

T. vaginalis

Causes Strawberry cervix

T. vaginalis

Diamond's modified culture medium

T. vaginalis

Feinberg Whittington culture medium

T. vaginalis

Trypticase Liver Serum Medium

T. vaginalis

Incubation of culture media for T vaginalis

37degree celsius

Pathox of T vaginalis to men

UTI


PROSTATITIS

Tissue protozoan form:



Longer Undulating Membrane (+) Volutin granules

Trypomastigote

Form of Tissue Protozoan:


Leishmanial


(-)Flagellum


Tx invading form

Amastigote

Form of Tx Protozoan:



Leptomonad


(+) flagellum


Found in vector

Promastigote

Crithidial form of Tissue protozoa


Short Undulating Membrane

Epimastigote

Vector of T cruzi

Reduviid


Triatomine


Assasin


Kissing Bug

IS of D. fragilis

Trophozoite bec. it has no cyst form

Cutaneous Leishmaniasis

L. tropica

Resembles "Leprematous leprosy"

L. tropica

IS


DS


of L. tropica

IS: Promastigote


DS: Amastigote

American Leishmaniasis

L. braziliense

Espundia

L. braziliense

Bubas

L. braziliense

New World Leishmaniasis

L. braziliense

Tapir nose

L. braziliense

Erosion of the Pinna of ear

Chiclero Ulcer

Chiclero ulcer

L. braziliense

Visceral Leishmaniasis

L. donovani

Why Black Fever (L.donovani)

Methemoglobinuria

Hepatosplenomegaly

L. donovani

Kala azar or Dumdum fever

L. donovani

Fatal Leishmaniasis

L. donovani

Cutaneous Leishmaniasis

L. tropica

Habitat of leishmania: Skin

L. tropica

Vector of L. tropica

Sandfly (Phlebotomus spp.Lutzomiya spp.)

Specimen of Leishmaniasis

Infected tx

Needle Aspiration stained with Giemsa

Leishmania spp.

Culture: NNN

Leishmania spp

Dr. Montenegro Intradermal test

Leishmania spp.

IS


DS



OF LEISHMANIA SPP

Is: Promastigote


Ds: Amastigote (inside Macrophage)

New World Cutaneous Leishmaniasis

L. mexicana

West African Sleeping Sickness

T gambiense

Kerandel's sign

T gambiense

delayed sensation to pain

T gambiense

Halo at the bite site


Earliest sign of African trypanosomiasis

T gambiense

Enlargement of Cervical Lymph Node

Winterbottom sign

Winterbottom sign

T gambiense

Point of difference of West and East African Sleeping sickness

West: (+) Lymphadenopathy

East African Sleeping Sickness

T. rhodesiense

CNS involvement appear early

T. rhodesiense

IS


DS



of African Trypanosomiasis

Is: trypomastigote


Ds: epimastigote

African trypanosomiasis assoc with Myocarditis

T. rhodesiense

Trypanosome that is pathog'c to Animals

T. brucei brucei

Ressurecting drug

Eflornithine for trypanosomiasis

Chagas dse or Mega dse.

T. cruzi

unilatelar swelling of the eyelid

Romañas Sign

Romañas sign

T. cruzi

Vector of T. cruzi

Triatomine Bug


Reduviid


Assasin


Kissing bug

Metacyclic trypomastigote

IS of T cruzi

Diagnostic Stage of T cruzi

Trypomastigote in blood

Best Technique to identify Dientamoeba fragilis in stool?

Trichrome stained smear

Causes "Nursery School Outbreaks"

G lamblia


D. fragilis


Cryptosporidium

Karyosome of Troph has cluster of "Achromatic Granules"

Iodameoba butschlii


Iodamoeba williamsi

Cx of Acanthamoeba

BAP flooded with E.coli

Pathogenic determinant of G lamblia

Large Ventral sucking disc

It makes the G lamblia resistant to intestinal proteolysis

VSP (Variant Specific Protein)

G lamblia:



🔝Surfac Area


For absorption

Vili

Flagellate that is associated with Steatorrhea

G lamblia

Undulating Membrane: 2/3 of the Costa

T. tenax

UM: As long as the costa

T. hominis

Inclusion bodies of T. vaginalis

Siderophil Granules

UM: <1/2 of of the costa


Largest trichomonas

T. vaginalis

Causes Pingpong's Dse.

T vaginalis

Female: Burning sensation & Strawberry cervix

T. vaginalis

Cx media of T vaginalis

1. Modified Diamond Cx media


2. Feinberg Whittington media


3. Trypticase Liver Serum Medium

"Wheeping Lesions"

Leishmania braziliense

Cx Media for Tryp and Leish

NNN

Vector of T cruzi in the PH

Triatoma rubrofasciata

Media for T cruzi

Changs Medium

Largest parasitic protozoan

B coli

Reservoir host of B. coli

Pigs

Troph of B coli

Macronucleus: Kidney shaped, vegetative


Micronucleus: Dot-like, reproductive


Cyst of B coli

Refractive double wall enclosing cilia

Chatacteristic of T. cruzi

C shape