Aids

Front 1

p24

Back 1

capsid protein
binds cyclophilin A

Front 2

the three viral enzymes for HIV virus

Back 2

protease, reverse transcriptase, and integrase

Front 3

RNA genome contains what three genes that code for various viral proteins

Back 3

gac, pol, env

Front 4

p23 (vif)

Back 4

viral infectivity factor (vif)
overcomes inhibitory effect of unidentified host factor, promoting cell-free viral transmission

Front 5

vpu

Back 5

viral protein U
promotes CD4 degradation and influences virion release

Front 6

env (gp160)

Back 6

Envelope protein
1. cleaved in ER to gp 120 and gp41
2. gp120 mediates CD4 and chemokine receptor binding, while gp41 mediates fusion

Front 7

mediates CD4 and chemokine receptor binding, while gp41 mediates fusion

Back 7

gp120

Front 8

nef (p24)
(4 things)

Back 8

Negative Effector
1. promotes down-regulation of surface CD4 and MHC I expression
2. blocks apoptosis
3. enhances virion infectivity
4. progression to disease slowed significantly in absence of Nef

Front 9

Blocks apoptosis

Back 9

nef (Negative Effector) p24

Front 10

inhances virion infectivity

Back 10

nef (Negative Effector - p24)

Front 11

contains control regions that bind host transcription facotrs like NFkB, NFAT, Sp1, and TBP

Back 11

LTR (long terminal repeats)

Front 12

required for the initiation of transcription

Back 12

LTR

Front 13

contains RNA trans-acting response element (TAR) that binds Tat

Back 13

LTR

Front 14

gag (Pr55gag)

Back 14

Polyprotein processed by viral protease
breaks into capsid, nucleocapsid, matrix, and p6

Front 15

interacts w VPR; core protein, participates in terminal steps of virion building

Back 15

p6 ()

Front 16

encodes a variety of viral enzymes including PR RT and RNAse

Back 16

pol (polymerase)

Front 17

vpr

Back 17

viral protein R (p15)
1. promotes G2 cell cycle arrest
2. Facilitates HIV infection of macrophages

Front 18

facilitates HIV infection of macrophages

Back 18

vpr

Front 19

regulator of viral gene expression (p19)

Back 19

promotes nulcear export incompletely spliced viral RNAs

Front 20

binds cyclophilin A

Back 20

capsid (p24)

Front 21

RNA binding protein

Back 21

nucleocapsid (p7)

Front 22

undergoes myristylation that helps target Gag polyportein to lipid rafts, promoting virus assembly at cell surface

Back 22

Matrix (p17)

Front 23

tat (p14)

Back 23

transcriptional activator
enhances RNA Pol II mediated elongation of integrated viral DNA

Front 24

when the HIV virus enters - the genome is ?
it then changes to and how?

Back 24

RNA then changes intracellularly into Proviral DNA using reverse transcriptase

Front 25

what happens in the initial lymphoid infetion in HIV

Back 25

follicular hyperplasia - germinal centers merge w/ interfollicular areas

Front 26

needed for membrane penetration

Back 26

gp41

Front 27

In HIV infections cytokine stimulation such as TNF activates what process

Back 27

transcriptio of HIV genome and transport of viral RNAs to cytoplasm - remember the DNA from which this is transcribed is integrated

Front 28

Chronic T cell activation can have what three outcomes

Back 28

1. viral replication in infected CD4+ T cells and cell lysis
2. Activation of uninfected CD4+ cells and apoptosis
3. expression of HIV peptides on infected CD4+ T cells - killing of infected cells by virus specific CTLs

Front 29

possible acute HIV syndrome, wide dissemination of virus, and seeding of lymphoid organs happens in what week

Back 29

about 6

Front 30

when are the viral particles in plasma the most

Back 30

duriing acute HIV syndrome and after latency (when virus is expressed again)

Front 31

when is Anti-p24 Ab the highest

Back 31

during latency

Front 32

when are CTLs specific for HIV peptides the highest

Back 32

after onset of initial syndrome on

Front 33

cell mediated response to HIV is detectable by what week

Back 33

2 to 3

Front 34

humoral response peaks at week

Back 34

12

Front 35

yeast like fungus that cuases pneumonia in pts with aids

Back 35

pneumocystis carinii

Front 36

cross between endothelial cells and sm muscle cells that appear and Kaposi's

Back 36

spindle cells

Front 37

spindle cells in kaposi's sarcoma are stimulated by

Back 37

cytokines and growht factors secreted by HIV infected T cells or the tumor itself

Front 38

non hodgkin B cell lymphoma caused by the herpes virus

Back 38

Kaposi's sarcoma

Front 39

the most common extranodal site affected lymphomas and an AIDs syndrome

Back 39

CNS

Front 40

overcomes inhibitory effect of uidentified host factor, promoting cell-free viral transmission

Back 40

vif (viral infectivity factor - p23)

Front 41

gene for viral replication in non-dividing cells - arrests cells in G2 and helps with virus replication

Back 41

vpr

Front 42

influences virion release and promotes the degradation of CD4

Back 42

vpu (viral protein U )

Front 43

gene - mtransactivation of viral transcription

Back 43

tat

Front 44

lipid bilyaer of virus is derived from

Back 44

host

Front 45

promotes CD4 degradation and influences virion release

Back 45

vpu

Front 46

overcomes the inhibitory effect of unidentified cell free viral transmission

Back 46

vif (p23)
viral infectivity factor

Front 47

promotes nuclear export of incompletely spliced viral RNAs

Back 47

rev (p19)
regulator of viral gene expression

Front 48

enhances RNA Pol 11 mediated elongation of integrated viral DNA

Back 48

tat (p14)

Front 49

slowed significantly in its absence

Back 49

Negative Effector (p24)
on capsid
also : promotes down regulation of surface CD4 and MHC 1

blocks apoptosis

enhances virion infectivity

Progression to disease

Front 50

what gene is responsible for the replication of the HIV virus w/in macrophages

Back 50

VPR

Front 51

the "gate keepers" of the HIV transmisstion

Back 51

monocytes/macrophages

Front 52

explain the cell to cell spread in HIV
gp120 binds?

Back 52

fusion of infected and uninfeted cells to make giant cells (synctia) - the gp120 binds the CD 4

Front 53

two major targets of HIV

Back 53

immune system and CNS

Front 54

what 2 cytokines are thought to play a role in activating transcription of latent HIV in CD4 cells

Back 54

IL-2 and TNF

Front 55

explain how monocytes/macrophages are resevoirs of replication and infection

Back 55

They bud small amounts of virus w/ large numbers of virus particle often in intracellar vacuales but they are resistant to the cytopathic effect of HIV (unlike CD4+)

Front 56

how does the HIV virus make it to the brain

Back 56

w/in monocytes (so most CNS HIV is M-tropic)

Front 57

encodes PR (p10

Back 57

pol

Front 58

encodes RT

Back 58

pol

Front 59

encodes p32 (IN)

Back 59

pol