Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
26 Cards in this Set
- Front
- Back
|
Elecrophysiology is |
detection of electric currents and potentials
|
|
|
Extracellular electrophysiology is
|
recording electrode outside of cell
|
|
|
Intracellular electrophysiology is
|
recording electrode inside of cell
|
|
|
Patch-clamp electrophysiology is
|
recording electrode attached to membrane.
|
|
|
In electrophysiology, the reference electrode is in
|
bath solution.
|
|
|
In electrophysiology, the amplifier measures
|
potential difference between recording and reference electrode.
|
|
|
Deflection of synaptic potential close to synapse when measured by extracellular electrode is
|
negative deflection.
|
|
|
In the hippocampus, the cell bodies all lie in
|
pyr
|
|
|
For neurons, the current sink is
|
synapses
|
|
|
For neurons, the current source is
|
cell body.
|
|
|
If you stimulate synaptic transmission at the level of the current sink (synapses), and have the extracellular electrode at the synapse it
|
activates synaptic transmission and causes negative deflection.
|
|
|
If you stimulate synaptic transmission at the level of the current sink (synapses), and have the extracellular electrode at the cell body it
|
causes positive deflection.
|
|
|
Field potential recordings are
|
in-vivo extracellular recordings mainly contributed by synaptic currents.
|
|
|
Local field potential (LFP) recording is
|
Field potential in-vivo extracellular recording where electrode is in brain.
|
|
|
EEG recording is
|
Field potential in-vivo extracellular recording where electrode is on scalp
|
|
|
ECoG recording is
|
Field potential in-vivo extracellular recording where electrode is placed on brain surface
|
|
|
In Depth (LFP), all deflections are
|
synaptic transmission
|
|
|
The size of the synaptic signal is reduced
|
further away from the synapse.
|
|
|
For field potential recordings, ______ has the highest signal, while _______ has the lowest.
|
LFP, EEG
|
|
|
Single-unit in-vivo extracellular recording is
|
recording of action potentials from individual neurons
|
|
|
Multi-unit in-vivo extracellular recording is
|
recording of action potentials from multiple neurons with more than 1 electrode.
|
|
|
Voltage clamp allows you to
|
clamp voltage at a value and measure current.
|
|
|
In 2 electrode voltage clamps, the electrodes are
|
1. In cell and measures voltage, 2. Outside cell for control.
|
|
|
For voltage clamp, the differential amplifier measures
|
potential different between electrode in cell and reference electrode.
|
|
|
For voltage clamp, the feedback amplifier measures |
Difference in measured voltage to clamped/command voltage and injects or withdraws current to compensate.
|
|
|
For voltage clamp, the feedback amplifier current is
|
exactly equal in opposite direction to current flowing into cell. |
