Ahg

Front 1

AHG

Back 1

antiglobulin test
-detects IgG antibodies and complement proteins that have attached to the rbcs but have not resulted in agglutination

Front 2

AHG principle

Back 2

-animals injected with human antibodies, and produce anti-human globulins, or anti-c3b/c3d
-we harvest these anti human globulins and use these as reagents
-the ahg will bind to the Fc portion of sensitising antibodies on rbcs
-these form bridges resulting in agglutination

Front 3

Coombs control cells

Back 3

-commercially prepared rbc with igG attatched
-added to all negative ahg tests
-reactions 2+ or stronger

Front 4

Polyspecific reagents

Back 4

-contains antibodies to human IgG and C3
-primarily used in DAT

Front 5

Monospecific Reagents

Back 5

-has either Anti-IgG or Anti-C3 only

Front 6

DAT

Back 6

Direct Antiglobulin Test
-detects IN VIVO bound antibody
-Hemolytic Disease of Newborn, transfusion
-Autoimmune Hemolytic Anemia
-Drug induced hemolytic anemia

Front 7

IAT

Back 7

Indirect Antiglobulin Test
-detects IN VITRO bound antibody
-need serum