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11 Cards in this Set

  • Front
  • Back
Blood agar
General purpose
pattern of hemolysis can aid in ID
Chocolate agar
(name 2)
blood was heated to lysis to release nutrients
-Haemophilus and Neisseria
Thayer Martin
(name 1)
(selective chocolate agar) Has antibiotics and selects for N. gonorroeae
Mannitol-salt agar
(name 1)
High salt con'c
-inhibits most organisms except S. aureus
-fermentation changes from red to yellow
MacConkey agar
Selective for gram -
(bile salts + crystal violet inhibits gram + and some fastidious -)
-Lactose is sole carb, fermention changes to pink/red, if not clear
Eosin Methylene Blue (EMB)
similar to MacConkey (except different dyes)
-lactose fermenters changes to blue/black w/ metallic sheen, if not clear
Bile esculin agar
(name 2)
bile salts inhibit gram + and most streptococci except Group D streptococci and enterococcus
<esculin can be hydrolyzed to esculitin which interacts w Fe to produce black precipitate>
Hektoen enteric (HE)
(name 1)
selective + differential for gram - (bile salts and indicator dyes inhibit gram +)
-sugars: lactose, sucrose, and salicin) ferments into yellow-pink, non is green/transparent
-if H2S is produced (salmonella) black precipitate
Cetramide
(name 1)
selective and differential for Pseudomonas aeruginosa, inhibits most bacteria as a detergent
-pigments: pyocyanin (blue-green), fluroescein (yello-green)
Tellurite (Cysteine tellurite)
selective and differential for corynebacterium diptheriae
-K tellurite inhibits gram - and most upper respiratory flora
-colonies appear gray-black (tellurite reductase activity), with brown halo (cystinase activity)
chromogenic
selective and differential for lots of bacteria (based on color; used to identify species or phenotypes -specific and quicker growth