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28 Cards in this Set
- Front
- Back
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steps to formation of callus bridge |
1.first cell division (24 hours) 2.callus bridge formation (5 days) 3.differentiation (10-15) 4.occurrence of vessels(15-20) 5.lignification of callus(25-45) |
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Symptoms of graft incompatibility |
1. few or no successful unions 2. successful unions that later die 3. nutrient deficiencies 4. dwarf trees 5. differential growth near union 6. degeneration of tissues |
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Types of graft incompatibility |
1.Non translocatable 2.Translocatable -Rootstock sends PGRs that are no bueno 3. Pathogen induced incompatibility -Virus/ phytoplasma |
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why wrap it |
humidity keep in place |
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what is callus tissue |
undifferentiated thin walled parenchema |
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photomorphogenesis |
physiological reaction to light |
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What made MS so boss |
addition of NO3 and NH4 allowed for more control of pH and better uptake of nutrients |
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difference b/t WPM and MS |
Less salts in WPM and lower chloride calcium chloride in MS replaced with calcium nitrate potassium nitrate in MS replaced with potassium sulfate |
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PGRS source and sink |
often moved in phloem, but can have local effects |
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Auxin |
polar transport shoot to roots promotes adventitious rooting |
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Cytokinin |
promotes cell division in the presence of auxin |
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gibberillins |
Cell elongation |
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ABA |
regulates stomatal opening drought stress |
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Ethylene |
accelerates ripening/ abscission |
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Why buy tissue culture propagated plants |
Only source virus free certified rapid introduction to the market place |
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stages of commercial plant microprop |
1. analysis 2. isolation 3. stabalization -obtain uniform and continuous shoots - obtain uniform consistant embryos or organs 4.optimization 5. rooting/ acclimatization |
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Adventitious systems |
embroygenesis organ culture shoot organogenesis |
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microprop advantages |
small space independent of seasonality little attention between subculture |
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new isolations problems |
erratic growth distorted leaves excess callus phenolic exudation |
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why cytokinin curve |
rate that only axillary buds compo axiliary adventitious toxicity -promote good shoot prolif from auxillary but not adventitious |
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ex vitro rooting |
more successful/ functional roots culture space |
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rejuvenation of episodic |
serial sub-culturing |
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juvenility |
inability to flower ability to form adv roots continuous growth (episodic plants) may involve dramatic shift increased runners |
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min somaclonal variation |
proper hormone levels (lowest level acceptable) minimize number of subcultures |
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reduce hyperhyd |
lower cytokinin increased ventilation adjust conc anf type of gelling material |
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why embryo rescue |
-shitty endosperm -complicated dormancy |
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physio of hyper |
lower stomata density less chlorophyll higher water malformed chlorplasts |
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causes of hyperhy |
high humidity high water potential in medium high cytokinin |
