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34 Cards in this Set
- Front
- Back
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What is the symmetry of the capsid?
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The capsid has an eicosahedral symmetry
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Is the capsid naked or enveloped?
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The capsid is naked.
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What is the name and the purpose of the fibers on the capsid?
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The capsid has "antenae" which are the fibers protruding off it and the purpose is to facilitate the binding on to the host.
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What is the purpose of the knobs on the antenae?
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The purpose of the knobs on the antenae are to bind the receptor on the target cells. :)
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How does the virus enter the host?
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The virus enters the host in endosome.
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What are the primary and secondary receptors?
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The primary receptor (in most cases) is the CAR [coxsackie & adenovirus receptor]
The secondary receptors are integrins, facilitating clathrin coated pit mediated endocytosis. |
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What are most regions of the genome transcribed by?
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cellular RNAPoly II
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Are adenoviruses known to cause cancer in humans?
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NO
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Adenoviruses are divided into groups [A-F] how are the groups classified?
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the classification is based on the ability to agglutinate blood.
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How does the virus exit the host?
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the virus exits the host via endosomes via a pH dependent mechanism.
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During cytosolic transportation to the nuclear pore, how does transpotation and interaction occur?
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transportation occurs via microtubules & interactions occur between the hexon and the microtubles.
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What happends once the capsid is at the nuclear pore?
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once at the nuclear pore, capsid disassembles and releases dsDNA genome into the nucleus.
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What does alternative splicing yield?
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alternative splicing yields a variety of translatable mRNAs
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Does different promoters yield the same mRNA?
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NO! Different promoters yield different mRNAs
[this is why from only 13 transcriptional units you end up w/ 50 different viral proteins] |
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GENOME ENCODES FOR RNA THAT NEVER GET TRANSLATED
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VA genes never get translated
[VA means "virus associated"] |
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GENOME ENCODES FOR RNA THAT NEVER GET TRANSLATED
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these genome segments are transcribed by cellular RNA Pol III
[this enzyme is normally used for tRNAs and other small RNAs] |
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GENOME ENCODES FOR RNA THAT NEVER GET TRANSLATED
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VA encoded RNAs inhibit PKR
--- PKR (ds RNA dependent protein kinase) |
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What does PKR inhibition avoid?
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PKR inhibition avoids destruction of the vius
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How many transcriptional promoters and poly(A) signals?
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8 transcriptional promoters and 12 Poly(A) signals
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How may early transcription units?
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6 early transcription units: E1A, E1B, E2A,E2B, E3, & E4
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There are 2 intermediate IVa2 and IX
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2 intermediate IVa2 and IX
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What is PKR INVOLVED IN?
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pkr IS INVOLVED IN INTERFERON SIGNALING
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2 mRNA exist that encode for E1A, how many amino acids do the first and second protein yield?
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1st = 289 aa
2nd = 243 aa |
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What type of cells do adenovirus target?
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Adenovirus targets non-dividing fully differentiated epithelial cells.
[proteins that aid in DNA rep have to be induced & E1A induces cells into S phase] |
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Is E1A oncogenic in experimental models?
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YES
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How does E1A work?
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it interacts with Rb & the Rb family
[normally Rb is interacting with E2F transcription factor] |
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Pap virus: is the genome associated with cellular histones?
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yes! and the genome size is 8 kbp
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What strains are associated with high risk of cervical cancer?
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Strains 16,18, & 31
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Which type of HPV target the feet?
a) 2 b) 7 c)9 d)1 |
D) HPV-1 taget feet
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Which type of HPV target hands
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HPV 2,4,& 7 targets the hands
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What are cervical warts referred to?
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Condylomas (which are benign and rarely progress to cancer)
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HPV 16 is of interest due to oncogenic properties. how many early genes are there?
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6 early genes: E1, E2, E4,E5, E6, E7
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HPV
infection requires entry to what type of cell? |
NON-DIFFERENTIATED basal cells
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HPV
what does the virus bind to? |
the virus binds to heparin and alpha 6-integrin
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