Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
20 Cards in this Set
- Front
- Back
|
Nucleation, Elongation, Steady State
|
What are the 3 steps in Actin polymerization
|
|
|
Actin Related Protein
|
Arp2/3 stands for
|
|
|
Formin
|
Protein dimer with FH2 actin binding domains
|
|
|
Spire
|
Linear protein associated with actin monomers to start nucleation
|
|
|
Thymosin
|
Prevents addition of actin by binding in the nucleotide binding pocket of actin. Keeps actin in ADP form
|
|
|
Profilin
|
Activated by VASP to cause polymerization of actin
|
|
|
Fimbrin, Alpha-Actinin, Villin
|
Some bundling proteins for stabilizing actin filaments are...
|
|
|
Spectrin, Filamin
|
Some matrix binding proteins are...
|
|
|
Calponin homology-domain
|
Crosslinking proteins contain CH domains. What are CH domains called
|
|
|
CapZ and Tropomodulin
|
Examples of capping proteins
|
|
|
Gelsolin and Cofilin
|
Examples of Severing proteins
|
|
|
Myosin
|
One way to see polarity of polymerization is by using
|
|
|
Chymotrypsin and Papain
|
Peptidases used to cleave myosin
|
|
|
Actin Assembly-inducing protein
|
ActA stands for
|
|
|
Biomimetic Systems
|
Using non-biological structures to see different biological processes
|
|
|
Vasodilator Stimulated Phosphoproteins
|
VASP stands for
|
|
|
Yeast 2-hybrid assay
|
Tool used to see how 2 proteins interact
|
|
|
1) Collect mRNAs 2) Reverse transcribe mRNAs into DNA 3) Screen each yeast colony for expressed protein
|
3 Steps to Yeast 2-hybrid assay
|
|
|
LaXp180
|
Protein found to cause asymmetric polymerization
|
|
|
Wiskott Aldrich Syndrome Protein
|
WASP stands for...
|
