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8 Cards in this Set
- Front
- Back
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adsorption chromatography
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A solid stationary phase and a liquid or gaseous mobile phase are used. solute is adsorbed on the surface of the solid particles. more strongly adsorbed => travels slower through column.
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partition chromatography
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A liquid stationary phase is bonded to a solid surface, which is typically the inside of the silica chromatography column in gas chromatography. solute ions of the opposite charge are attracted to the stationary phase by electrostatic force. mobile phase is liquid.
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ion-exchange chrom.
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anions such as sulfite or cations such as N(CH3)3+ are covalently attached to the stationary solid phase, usually a resin. solute ions of opposite charge attracted to stationary phase by electrostatic force.
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molecular exclusion chrom.
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aka gel filtration/permeation. separates molecules by size, larger solutes passing through quickest.
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affinity chrom
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selective. specific interactions bt one kind of solute molecule and a second molecule that is covalently attached to the stationary phase.
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volume flow rate
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mL per minute through column
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linear flow rate
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how many cm traveled by solvent in one minute
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retention volume
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volume of mobile phase required to elute a particular solute from column.
=retention time * volume flow rate |
