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6 Cards in this Set
- Front
- Back
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What is DNA ligation? What is an example of a clinical application?
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Joining linear DNA fragments together with covalent bonds
Example: attachment of human genes to plasmids |
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Two fragments of DNA can be linked to form what?
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Recombinant DNA
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What is transformation? What is an example of an application where transformation is used?
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The genetic alteration of a cell resulting from uptake and expression of foreign material
Example: Inserting a recombinant a plasmid into a bacteria |
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What is the difference between therapeutic cloning, reproductive cloning, and embryo cloning?
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Therapeutic: a specific organ or tissue is produced from a stem cell
Reproductive: an animal is produced that is genetically identical to the host Embryo: several genetically identical animals are produced |
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How is a transgenic animal made?
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A foreign gene is inserted into a one-cell embryo
Transgene is then represented in every cell of the animal |
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What is the difference between Southern, Northern, and Western DNA sequence hybridization?
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Southern hybridization can identify
Northern hybridization can identify an RNA sequence Western hybridization can identify proteins expressed after translation |
