Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
13 Cards in this Set
- Front
- Back
|
What initiates protein synthesis?
|
Activated by GTP hydrolysis, initiation factors help assemble the 40S ribosomal subunit with the initiator tRNA
|
|
|
What is the first step of protein synthesis?
|
Aminoacyl-tRNA binds to A site
|
|
|
What is the second step of protein synthesis?
|
Ribosomal rRNA catalyzes peptide bond formation, transfers growing polypeptide to amino acid in A site
|
|
|
What is the third step of protein synthesis?
|
Ribosome advances 3 nucleotides toward 3' end of mRNA
|
|
|
What does the APE mnemonic for protein synthesis?
|
A - incoming aminoacyl-tRNA
P - accommodates growing Peptide E - holds Empty tRNA as it Exits |
|
|
What do aminoglycosides inhibit?
|
Bind 30S and inhibits formation of initiation complex
|
|
|
What do tetracyclines block?
|
Bind 30S and block aminoacyl tRNA from entering acceptor site
|
|
|
What does chloramphenicol inhibit?
|
Binds 50S and inhibits peptidyl transferase
|
|
|
What do macrolides prevent?
|
Bind 50S and prevent release of uncharged tRNA
|
|
|
What types of subunits do eukaryotes have?
|
40S + 60S = 80S (Even)
|
|
|
What types of subunits do prokaryotes have?
|
30S + 50S = 70S (Odd)
|
|
|
What is the trimming of protein?
|
Removal of N- or C- terminal propeptides from zymogens to generate mature proteins
|
|
|
What is proteasomal degradation?
|
Attachment of ubiquitin to defective proteins to tag them for breakdown
|
