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14 Cards in this Set
- Front
- Back
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What is a large contributor to the cell glycocalyx?
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Sugar chains on the surface areas of integral membrane proteins.
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What are 2 proteins that associate with thte cytosolic surface of the cell as well as the membrane skeleton?
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-Ankyrin
-Spectrin |
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What is the Pface?
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Protoplasmic - cytoplasmic
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What is the E face?
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Extracellular
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What is FRAP?
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Flourescence recovery after photobleaching
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What does FRAP allow you to determine?
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Diffusion coefficient - after bleaching a spot, how long it takes for flour to get redistributed.
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What has the highest lateral mobility diffusion coefficient in the membrane?
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Phospholipid > Rhodopsin > Membrane proteins
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How do you study the RBC membrane?
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By putting it in hypotonic soln to get just a ghost.
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What method is used for analysing the Ghosts of RBCs?
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SDS-PAGE
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What did early SDS-Page analysis of RBC ghosts identify?
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BAND proteins
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What are the 2 principle membrane proteins in the RBC?
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-Glycophorin
-Band 3 (anion channel) |
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What are the 4 principle membrane proteins that make the membrane skeleton?
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1. Spectrin
2. Actin 3. Ankyrin 4. Band 4.1 |
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Why aren't integral membrane proteins as laterally mobile as phospholipids?
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Because they are anchored to the cytoskeleton
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What disease results from abnormalities in membrane cytoskeletal proteins?
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Hereditary spherocytosis
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