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14 Cards in this Set

  • Front
  • Back
What is a large contributor to the cell glycocalyx?
Sugar chains on the surface areas of integral membrane proteins.
What are 2 proteins that associate with thte cytosolic surface of the cell as well as the membrane skeleton?
What is the Pface?
Protoplasmic - cytoplasmic
What is the E face?
What is FRAP?
Flourescence recovery after photobleaching
What does FRAP allow you to determine?
Diffusion coefficient - after bleaching a spot, how long it takes for flour to get redistributed.
What has the highest lateral mobility diffusion coefficient in the membrane?
Phospholipid > Rhodopsin > Membrane proteins
How do you study the RBC membrane?
By putting it in hypotonic soln to get just a ghost.
What method is used for analysing the Ghosts of RBCs?
What did early SDS-Page analysis of RBC ghosts identify?
BAND proteins
What are the 2 principle membrane proteins in the RBC?
-Band 3 (anion channel)
What are the 4 principle membrane proteins that make the membrane skeleton?
1. Spectrin
2. Actin
3. Ankyrin
4. Band 4.1
Why aren't integral membrane proteins as laterally mobile as phospholipids?
Because they are anchored to the cytoskeleton
What disease results from abnormalities in membrane cytoskeletal proteins?
Hereditary spherocytosis