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14 Cards in this Set
- Front
- Back
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picornavirus are small ____ (genome) containing viruses. What does the genome look like? What is different about it's 5' end?
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- + RNA
- long open reading frame - makes polyprotein - has VpG at 5' end (viral protein) |
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What are next steps after picornavirus makes it's polyprotein? What proteins are encoded?
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- polyprotein is cleaved - makes new enzyme replicase which can replicated genome - not present before infection
- protease - VpG - which can now associated with income mRNA as a primer - Vps |
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How does the piconavirus replicate?
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1) VpG protein is the primer
2) make - RNA b/c more efficient template --> make tons of + strands off of - RNA |
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Myxovirus has a ______ genome. Is it segmented or polyprotein?
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- (-) RNA
- segmented genome |
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What happens when myxovirus first infects cell? What is made and what inserts into membrane?
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- synthesies + mRNA from - RNA
- formation of RNP & insertion of HA & NA into membrane - budding & release |
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The (ortho) myxoviruses are subdivided by antibody of surface proteins _____ & ______ into serotypes ____, ____, etc.High level of genetic mixing (recombination) to generate novel serotypes associated with occasional_______.
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- hemagglutinin
- Neuraminidase (NA) - H1N1, H2N2, etc - pandemics |
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What is unique about the myxovirus mRNA?
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- no polyA tail or 5' cap
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What happens when you add hemagglutinin (HA) to RBCs? What does it recognize?
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- they will all be stuck together
- this is the receptor binding recognizes salicylic acid or neuramic acid |
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What does neuraminidase do?
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- cleaves the receptor so it can no longer be used by the virus - needed when escaping the cell to go infect others
- cleaving neuramic acid/salicylic acid |
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How can antibody be used for an agglutination assay? What happens if you add NA?
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- if you add antibody then it should bind to HA & RBCs should not aggregate
-if you add HA then add NA they should not aggregate anymore |
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Antibodies to NA & HA in our body do what?
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- protect us against the infection
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What are the early events in myxovirus infection?
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Adsorbtion via interaction of HA with sialic acid on cell surface
Penetration by receptor-mediated endocytosis pH triggered fusion and uncoating Transport of RNA to nucleus |
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What are the steps of myxovirus transcription?
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Synthesis of (+) mRNA using 5’ caps stolen from cellular mRNA as primer
Transport of mRNA to cytoplasm |
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What viral protein in myxovirus decides whether to make + RNA & - RNA?
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- NP protein - tells it to make +RNA & replicate genome & go make HA & NA
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