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27 Cards in this Set
- Front
- Back
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Homogenous ANA pattern is highly suggestive of:
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SLE
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Which antibodies cause a positive homogenous pattern?
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-Anti-histone
-Anti-ribonucleoprotein |
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Antigens detected in a homogenous pattern are:
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dsDNA, ssDNA, nDNA, DNA, and
Histone. |
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Rim ANA pattern is highly suggestive of:
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SLE
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Which antibodies cause a RIM pattern?
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Anti-dsDNA
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Which cells exhibit the rim pattern, mitotic or non?
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Non-mitotic.
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Antigens detected in a rim pattern are:
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same as in homogenous; dsDNA, ssDNA, nDNA, DNA, and histone.
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What is the most common ANA pattern?
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Speckled
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What are high titers of speckled ANA cells suggestive of?
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SLE, Scleroderma, Sjogren's, and MCTD.
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Antigens detected in a SPECKLED pattern are:
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Sm, RNP, Scl-70, and SS-B.
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What is the least common ANA pattern?
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Nucleolar
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How do nucleolar cells look?
-Mitotic -Nonmitotic |
Mitotic - negative
Nonmitotic - only nucleoli flouresce. |
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A high titer of nucleolar staining is suggestive of:
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Scleroderma
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Antigens detected in a NUCLEOLAR pattern are:
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4-6s and RNA
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After getting a pos ANA of a particular pattern, what is the next step?
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Do specific testing based on which pattern is observed.
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What patterns could be observed for SLE?
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-Homogenous (most likely)
-Rim (likely) -Speckled |
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what are specific tests for SLE?
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1. Anti-native DNA (dsDNA)
2. Anti-Sm 3. Anti-RNP |
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What methodology can be used for all specific autoab testing?
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ELISA/EIA
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What additional methods can be used for SLE-spcf testing?
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IDA
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What is a specific test for MCTD?
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Anti-RNP in HIGH titers.
Methods are IDA, Elisa/EIA. |
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What are specific tests for Sjogren's Syndrome?
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1. Anti-SS-A
2. Anti-SS-B -IDA, Elisa/EIA |
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What is a specific test for drug-induced SLE?
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Anti-histone by IDA/ELISA/EIA.
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What is a specific test for Scleroderma?
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Anti-Scl-70 by IDA/Elisa/EIA.
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What test is specific for Myositis?
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Anti-Jo-1 by IDA/Elisa/EIA.
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What test is specific for RA?
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Rheumatoid FACTOR (rf) by Latex agglutination, Nephelometry, and Elisa/EIA of course.
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What isotype antibody is RF?
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Usually IgM (but can be IgG)
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What is the RF antibody against?
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Anti-Fc portion of IgG/
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