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34 Cards in this Set
- Front
- Back
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Micro organism |
Living things to small to he seen with a naked eye |
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Ubiquitous |
Found everywhere or ever present |
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Contamination |
Unintentional exposure to microbe |
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Inoculation |
Intentional exposure to microbe |
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Fomite |
object or surface that can harbor and transfer microbe |
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Culture |
Growth of microbes in lab |
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Sabouraud Dextrose Agar |
Classification -selective media Encourage the growth of certain microbe and prevent others from growing Low ph high sugar/grows fungi |
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Nutrient agar |
Classification-Standard media Basic mix of nutrient to grow microbe |
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Cryptic soy agar |
Classification-enriched media Includes extra nutrient for growth of fastidious bacteria(picky eater) Grows microbe from human skin |
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Two goals of aseptic technique |
Protect yourself Protect your patient/experiment |
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Staining False positive |
Looks like purple should be pink
Forgot decolorizer/ used too little
Wrong decolorizer (H2O)
Mordant left on for too long
Too much bacteria on slide
Wrong secondary stain |
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Staining False negative |
Looks like pink should be purple
Decolorizer left on for too long/ used too much Wrong decolorizer (acid alcohol) Forgot mordant/ used too littleForgot primary stain/ used the wrong primary stain |
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Special stains |
Used to observe one structure in the bacterial cell • Three types: - Endospore stain ✔ - Capsule stain ✔ - Flagella stain X |
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Capsule |
clear halos around the stained bacterial cell |
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Primary stain |
Malachite green • Cover smear with paper towel and add dye on top |
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Mordant |
*Heat* • Place slide on hot plate for 5 min (keep moist by adding more dye as needed) |
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Decolarizer |
DI H2O • Rinse well • No such thing as too much water |
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Secondary stain |
Safranin • 45 sec • rinse with DI H2O |
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Endospore stain |
Green = endospores (inside and outside bacterial cells) • Red = vegetative bacterial cell |
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Spread plate |
Used to quantify bacteria |
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Streak plate |
Used to isolate bacteria |
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Biochemical testing |
Helps identify organisms on the basis of what they can metabolize or the enzymes they make • Utilizes a dichotomous key |
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Enterobacteriaceae family |
-Gram negative bacilli – Oxidase negative (can grow without oxygen) – Glucose fermenters |
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–Carbohydrate fermentation test (Durham tubes) |
Contain a small inverted test tube inside • Inoculate 1 Glucose durham and 1 Lactose durham |
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Indolence test |
clear broth containing trypton |
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–MR-VP à yellowish broth |
MR = Methyl red • VP = Voges-Proskauer
Test for waste products of glucose fermentation (acidic waste or neutral waste) Only one test, MR or VP, can be positive |
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Triple sugar iron (tsi) |
Red/orange colored slant • Streak slant with a loop and stab butt with a needle |
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Citrate test |
Green slant |
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Grease test |
Beige slant |
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–Eosin Methylene Blue (EMB) |
Burgundy color |
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Maconkey plate |
Rose color
incubate at room temp in designated bin |
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TSA plate |
Beige color |
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MR test |
Add 5 drops of methyl red - Red broth = (+) for acidic waste from glucose fermentation |
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Vp test |
Add 10 drops reagent A and 5 drops reagent B (wait 45 min) - Red broth = (+) for neutral waste (acetoin) from glucose fermentation |
