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44 Cards in this Set
- Front
- Back
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Mycoplasma morphology
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Mycoplasma morphology:
- no cell wall: G stain not good, use Giemsa - very small: pass through filters, hard to see - pleomorphic: rings, spirals, filaments |
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Mycoplasma colonies
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Mycoplasma colonies:
- minute - soft agar: 20% serum, cholesterol (for cell mem), Pen, thallium acetate (to inhibit regular bacti) - 3-7 days 5-10% O2: minute fried-eggs as center digs into agar |
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Mycoplasma ID
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Mycoplasma ID:
1. colony Diene's stain 2. colony FA stain: specific 3. growth inhibition by specific antiserum 4. serology: (Ab) ELISA, agglutination, hemagglutination inhibition |
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Mycoplasma virulence
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Mycoplasma virulence:
1. attachment to mucosa via: capsular material (galactan in M. mycoides), contractile protein ( M. gallisepticum) 2. cytotoxins, endotoxins in some 3. hydrogen peroxide: cell injury 4. metabolic competition: some deplete Arg= dec lymphocyte fx 5. surface Ag variation |
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Mycoplasma dz
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Mycoplasma dz:
- endo or exogenous: aerosol, venereal, vertical ( in utero/ egg) - pathogenesis: host stress, viral/bacti infection, poor ventilation - dz: respiratory, jts, mammae, urogenital |
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Mycoplasma tx, control
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Mycoplasma tx, control:
1. TETRA, tylosin, erythro, tiamulin, lincomycin, spectinomycin, enrofloxacin 2. vaccs: don't eradicate - poultry: live M. gallisepticum - pigs: M. hyopneumoniae bacterin - contagious bovine pleuropneumonia: live 3. admit only SPF animals in dz free herds, flocks |
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Mycoplasma habitat
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Mycoplasma habitat:
- mucosa - mostly host specific - environmental survival short |
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Mycoplasma bovine mastitis
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Mycoplasma bovine mastitis:
- contagious agalactia: M. bovis, rarely other spp - dec, abnormal milk - high SCC - ID, cull infected: tx not effective |
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Mycoplasma bovine genital inf
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Mycoplasma bovine genital inf:
- mainly M. bovis, but also bovigenitaleum, canadense, ureaplasma - aberrant estrus cycles - less common: vaginitis, endometritis, seminal vesiculitis - antibiotics (minocycline) semen extender - intrauterine instillation of Tetra |
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Mycoplasma bovine arthritis
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Mycoplasma bovine arthritis:
- M. bovis: USA, Can - capable of systemic invasion: arthritis, pneumonia |
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Mycoplasma bovine pneumonia
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Mycoplasma bovine pneumonia:
- M. dispar - calves - progressive w/ Pasteurella, Mannheimia |
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Mycoplasma contagious bovine pleuropneumonia dz
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Mycoplasma contagious bovine pleuropneumonia (CBPP):
- endemic: Africa, Asia, Middle East, Meditteranean - M. mycoides mycoides 1. lung congestion, consolidated lobules, thickened interlobular septae 2. blood-tinged pleural fluid, fibrin 3. fibrin deposition, marbling |
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CBPP signs
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CBPP signs
1. high fever 2. labored breathing, head extended 3. mucopurulent nasal discharge 4. possible death: 1-3 weeks |
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M. agalactae small R
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M. agalactae small R:
- worldwide, esp Europe 1. severe mastitis: dec milk, possible complete fibrosis 2. septicemia 3. death: 20% effected animals 4. arthritis in survivors - tetra, control via culling |
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M capricolum capripneumoniae gen
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M capricolum capripneumoniae:
- contagious caprine pleuropneumonia - Africa, Middle East - agent: fragile, difficult to grow, isolate - incubation ~10 days - goats of all ages |
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contagious caprine pleuropneumonia signs
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contagious caprine pleuropneumonia signs:
1. fever 2. accelerated resp, extended neck, violent coughing 3. continuous salivation, mucopurulent nasal discharge 4. inability to move 5. death - subacute, chronic: milder signs 3. |
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contagious caprine pleuropneumonia pathology
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contagious caprine pleuropneumonia pathology:
1. hepatization, adhesion, pleuritis, accumulation of pleural fluid 2. pleural exudate can solidify to form a gelatinous fibrin covering on lung 3. NO thickening of interlobular tissue (unlike CBPP) 4. granular, liver-like lungs |
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CCPP dx
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CCPP dx:
- frozen pleural fluid, section of hepatized lung at interface of normal/dz tissue +serum to lab (in ice if <2 days) 1. special Mycoplasma media 2. PCR: dried pleural fluid on paper 3. serology: latex agglutination |
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CCPP prevention, control
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CCPP prevention, control:
1. affected, in contact: dx, slaughter ~~~endemic areas~~ 1. vaccs: sonicated Ag emulsified in adjuvant - strain inactivated w/ saponin 2. antibiotics: Tetra, tylosin, tiamulin - shouldn't be used in eradication |
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Mycoplasma (Eperythrozoon) ovis
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Mycoplasma (Eperythrozoon) ovis:
- ovine eperythrozoonosis - worldwide: arthropods poss involved - lambs: fever, anemia (parasitized RBCs), enlarged/ soft spleen - ewes may carry |
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M. hyopneumoniae dz
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M. hyopneumoniae:
- enzootic pneumonia: worldwide, all ages of swine - predisp: poor vent, crowding - coughing, poor growth - PM: consolidation of apical, cardiac lobes |
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M. hyorhinitis dz
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M. hyorhinitis:
- young pigs (<10 weeks): chronic polyserositits, arthritis - fever, labored breathing, lameness, swollen jts - PM: serofibrinous pleurisy, pericarditis, peritonitis |
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M. hyorhinitis dx, tx
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M. hyorhinitis:
- dx: lesions, culture, rule out Hemophilus suis and Strep suis - Tylosin, lincomycin (early admin) |
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M. hyopneumoniae dx, tx
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M. hyopneumoniae:
- dx: FA sample , culture - Tylosin, Tiamulin, Lincomycin - vaccs: poor protection - prevent: dev of SPF herds |
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M. hyosynoviae
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M. hyosynoviae:
- pigs 10-30 weeks: polyarthritis - culture ID - prevention: early weaning, prophy tylosin in feed of problem herds - no commercial vaccs |
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M. gallisepticum dz
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M. gallisepticum:
- worldwide 1. chronic respiratory dz: chicken - nasal dis, tracheal rales, coughing, sneezing 2. infectious sinusitis: turkey - swelling of paranasal sinuses, mild conjunctivitis - dec growth, egg production |
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M. gallisepticum dx, tx
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M. gallisepticum:
- dx: culture, HI on serum - tetra in feed, tylosin in water - eggs: tylosin, heat to 46.1 C - vaccs: variable efficacy, partial protection |
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M. meleasgridis dz
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M. meleagridis:
- turkey pathogen: worldwide, egg transmitted - dec hatchability, air sacculitis in poultry - lameness: poor growth, skeletal abnormalities |
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M. meleasgridis dx, tx
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M. meleasgridis:
- culture, confirm by FA - serology: plate agglutination - tiamulin:water for 1st 10 says life - use M. m free egss: egg dipping - No vaccs |
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M. synoviae dz
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M. synoviae dz:
- poultry: infectious synovitis - aerosol transmission - lameness: synovitis, arthritis - resp: nasal discharge, rales |
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M synoviae dx, tx
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M. synoviae:
- dx: culture, serology - feed: Tetra - water: lincomycin, spectinomuycin - dev: SPF stocks - acquire stock from M. s free flocks |
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Mycoplasma present in healthy dogs
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Mycoplasma present in healthy dogs but may cause dz:
- M. cynos: kennel cough complex - M. canis: endometritis, infertility - Ureaplasma spp: poss infertility |
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M. hemocanis (Hemobartonella canis) dz
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M. hemocanis (Hemobartonella canis) dz:
- infectious, progressive anemia: splenectomized or immunocomp dogs - tick transmission poss - lethargy, anorexia, weight loss, fever - death in some cases - - |
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M. hemocanis (Hemobartonella canis) dx, tx
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M. hemocanis (Hemobartonella canis):
- dx: coccoid org seen on RBCs in stained blood, PCR - isolation by culture difficult - acute cases: doxy |
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M. felis
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M. felis:
- conjunctivitis: young cats - poss role in arthritis, resp dz |
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M. gatae
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M. gatae:
- cats - poss role in chronic arthritis, tenosynovitis |
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M. hemofelis (Hemobartonella felis) dz
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M. hemofelis (Hemobartonella felis):
- feline inf anemia: contagious, esp in free-roaming cats <3 y/o, esp males - inf: bite wounds, vertical trans - severe hemolytic anemia: death or chronic, weak carriers - prominent icterus |
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M. hemofelis (Hemobartonella felis) dx, tx
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M. hemofelis (Hemobartonella felis):
- dx: blood smear FA, PCR - non-culturable - tx: tetra, fluoros, blood transfusion |
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Horse Mycoplasma
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Horse Mycoplasma:
- dz uncommon 1. M. felis: pleuritis, pneumonia reported 2. M. equigenitalium: abortion |
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rodent Mycoplasma
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rodent Mycoplasma:
- pulmonis: resp dz |
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CBPP dx
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CBPP dx:
1. signs 2. pm lesions 3. FA of pleural fluid 4. ELISA 5. agglutination for Ab |
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CBPP diff dx
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CBPP diff dx:
- pneumonic - Pasteurella/ Mannheimia inf - hemorrhagic septicemia |
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CBPP prevention
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CBPP prevention:
1. before importation: serology (agglut, ELISA) 2. dz free areas: test and slaughter policy 3. tx: leads to carrier state, not recc 4. live vaccs: endemic areas, not USA, CAN |
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MAKePS in goats
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MAKePS in goats:
- worldwide - various Mycoplasma - TETRA 1. Mastitis: cannot be tx, cull 2. Arthritis 3. Keratitis 4. Pneumonia 5. Septicemia |
