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18 Cards in this Set
- Front
- Back
What is it? Where does it absorb |
NAD+ 260nm B/C of Ribose Ring (Middle Ring) 340 nm no absorbtion |
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What is it? where does it absorb |
NADH 260 for Ribose 340 nm for Pi System on top |
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What is it, What's it used for, Where does it absorb. |
Coomassie Brilliant Blue To determine amount of protien in sample, because it binds to proteins @ it's Nitrogens Blue Forme binds to proteins Red Form: 450 & ~660 Blue: 595 nm |
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What is it where does it absorb |
Tryptophan 280nm |
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What is it
where does it absorb |
Tyrosine 280 nm |
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What is it. what does it do |
IPTG: Isopropylthiogalactoside It acts as an inducer for our plasmids Can't be metabolized like Allolactose lolol |
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What is it what do we use it for |
SDS Sodium Dodecyl Sulfate Destroy all Secondary, tertiary, quartenary structure of all proteins Makes them rod like andstuff Gives them a slight negative charge too |
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Dithioltreitol (DTT) Reduces Disulfide Bonds |
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B-Mercaptoethanol (Beta-ME) Reduce/break disulfide bonds (reqiures 2 to use) |
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Bromophenyl Blue used as tracking dye. acts as very small protein that will run through the gel real quickly |
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Urea breaks 2ndary structures |
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Acrylamide Used for the gel |
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Bisacrylamide for sds gel |
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Ammonium Persulfate to start acrylamide polymerization |
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TEMED Tetramethylethylenediamine acrylamide polyerization |
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Tris Tris(hydroxymethyl) aminomethane Tris Buffer to keep pH stable in Acrylamide gel |
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5bromo 4Chloro 3indolyl phsophate X-P This is dephosphorolated by secondary antibody and dimerizes to form nitroblue tetrazolium |
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Nitroblue Tetrazolium Preciptate formed when we mark with secondary antibodies (the formazan forms that is coloured) |
