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22 Cards in this Set
- Front
- Back
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Sepsis |
refers to bacterial contamination, like in living tissue, or in a patient setting, sepsis is a severe bacterial infection. |
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Asepsis |
is the absence of significant bacterial contamination. Aseptic surgical techniques prevent the microbial contamination of wounds. Aseptic lab techniques prevent contamination of our lab cultures. |
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Sterilization |
removing and/or destroying all microbrial life (nothing is left alive) |
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Commercial Sterilization |
kiling Clostridium botulinum endospores from canned goods |
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Disinfection |
destroying harmful microorganisms. |
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Antisepsis |
destroying harmful microorganisms on living tissue |
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Lysol are? |
disinfectant |
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Listerine are? |
antiseptic |
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D-value is? |
the time needed to decrease a bacterial population by 90% |
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How various factors can affect the rate of microbial death? |
population size decreases by 90% every minute |
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Understanding the Microbial Death Curve |
plotting the typical microbial death curve logarithmically (red line) results in a straight line. |
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What are the Rate of Microbial Death |
number of microbes environment time of exposure microbial characteristics |
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What are the three basic ways a microbial control agent can affect a mircobe? |
Alteration of membrane permeability damage to proteins (enzymes) damage to nucleic acids |
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Alteration of membrane permeability |
stuff leaks in to or out of the cell |
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Damage of proteins (enzymes) |
breaking bonds leads to denaturation of proteins |
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Damage of nucleic acids |
damage to DNA or RNA prevents replication and sythesis of proteins |
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How heat affects microbes |
Heat denatures enzymes Thermal death point (TDP) Thermal death time (TDT) Decimal reduction time (DRT) |
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Thermal Deat Point (TDP) |
lowest temperature at which all cells in a liquid culture are killed in 10 minutes |
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Thermal Death Tim (TDT) |
minimal time for all bacteria in a liquid culture to be killed at a particular temperature |
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Decimal Reduction Time (DRT) |
minutes to kill 90% of a specific population of bacteria at a given temperature. Same as the D-value from a previews slide, but here it applies to heat treatment instead of chemicals |
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Autoclave |
steam under pressure to achieve a temperature > 100 degree C |
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Simple pressure cooker |
a home pressure cooker works by increasing the temperature at which water boils. This forces more heat into foods, allowing faster cooking times |
