Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
24 Cards in this Set
- Front
- Back
|
Electrophoresing DNA and hybridizing a probe to it is called
|
Southern blotting.
|
|
|
When electrophoresis of RNA is performed and subsequently hybridized to a probe, the technique is called
|
Northern blotting.
|
|
|
Artifacts may include wrinkles of the section (which may be confused with ?), precipitates of stain (which may be confused with ?)
|
blood capillaries, cytoplasmic granules
|
|
|
10^1 microns = ? nanometers = ? Angstroms
|
10^3, 10^4
|
|
|
About how many microns or angstroms are: a cell? A mitochondrion? Diameter of a dna double helix? A hydrogen atom?
|
cell ~15um, mitochondrion ~0.5um, DNA dbl helix diam ~20A, hydrogen atom ~2A
|
|
|
What is the resolution of : human eye? 10X magnifying glass? Compound microscope? TEM?
|
human eye ~0.2mm, 10x mag glass ~20um, compound microscope ~ 0.2um, TEM ~0.1nm
|
|
|
What is the “unit membrane?”
|
under a TEM, the two dense layers of plasma membrane separated by a lighter layer
|
|
|
What is the function of the nucleolus? Its different areas?
|
general function - ribosome production, fibrils - where DNA is transcribed, granules - site of ribosomal RNA assembly into ribosomes
|
|
|
What is the space between the two membranes of the nuclear envelope?
|
the perinuclear cisterna
|
|
|
A mitochondrion has two membranous sacs, the outer sac is ?, the inner sac is ? and forms ?
|
outer sac is smooth and highly permeable, inner sac is selectively permeable and folds to form cristae
|
|
|
What are the functions of the mitochondria?
|
ATP production, Ca++ storage, steroid production (side chain cleavage)
|
|
|
The Rough Endoplasmic Reticulum comprises sacs enclosing fluid filled cavities called ?
|
cisternae
|
|
|
RER-associated ribosomes are in the form of ?
|
polyribosomes
|
|
|
Proteins made by the rough ER are often, but not always, ?
|
exported from the cell
|
|
|
SER's known functions are
|
biosynthesis of steroids and membrane lipids, detoxification of lipid-soluble drugs, toxins and hormones, glycogen metabolism, calcium sequestration and release (sarcoplasmic reticulum)
|
|
|
What are the known functions of the Golgi complex?
|
post-translational modification of proteins, condensation and packaging of proteins, formation of lipoproteins, processing of acid hydrolases, formation of the acrosome in sperm, "sorting" of various proteins
|
|
|
Vesicles may be smooth or coated with ?
|
clathrin
|
|
|
Lysosomes contain ? and function? arise from?
|
acid hydrolases... intracellular digestion, autophagy, heterophagy, tissue remodeling...RER, Golgi
|
|
|
Peroxisomes are formed from the Golgi, true or false? They contain peroxidase, true or false?
|
false! false!
|
|
|
Peroxisomes are involved in ? via their two main enzymes ?? which ?
|
lipid metabolism, removal of hydrogen peroxide...oxidative enzymes, catalase...beta oxidation of long chain fatty acids, break down hydrogen peroxide to water and oxygen
|
|
|
Centrioles form centrosomes, which contain ? microtubules and the basal bodies of cilia, which contain ? microtubules
|
9 triplets, 9 doublets and two central singlets
|
|
|
List 5 non-organelle intracellular inclusions
|
pigment granules, secretory granules, glycogen, lipid droplets, crystals e.g. Reinke crystals in Lydig cells
|
|
|
What is the composition of the Ground Substance?
|
water, ions, metabolites, proteins, and molecules
|
|
|
How thick is the plasma membrane?
|
~8-10nm
|
