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8 Cards in this Set
- Front
- Back
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make a miRNA
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transcribe. makes hairpin. Drosha cuts. put out. dicer and helicase. RISC complex
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difference between miRNA and siRNA
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miRNA made from transcipts
small interfering siRNA are from dsRNA or shorthairpin shRNA |
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whats the big miRNA ;locus
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C19MC
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how do you stop a miRNA
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with an antagomir or anti-miRNA
masking oligonucleotides decoy sponge alternative sequence |
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modify siRNA's at which position
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2'. methyls or F
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what are aptamers
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short sequence that binds to specific things like protein- like an antibody
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long term impant of RNAi
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use shRNA via adenoassociated virus AAV. don't integrate (stay episomal) but last a while.
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example they gave for cholesterol
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antagomir against miR 122 which reduces DNA binding protein that inhibits txn of cholesterol biosyntheis genes. blocking a blocker of a blocker.
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