Why Study Protein Domains

Scientific experiments and observations have led to many discoveries, which in turn have allowed scientists to develop a better understanding on the way diseases originate in humans. Molecular biology in particular has allowed scientists to discover the way in which domains, found in proteins impact a genome. Domains are autonomous folding units that carry out specific functions and mechanisms in a certain protein. These functions consist of being responsible for interactions in the cell, which ultimately determines the role of the protein. Domains found in proteins can develop independently which in turn can change the role of the protein. Domains are able to develop independently through certain mechanisms. Some of these mechanisms include gene recombination and the duplication of a domain coding sequence. The way in which a domain evolves and is able to rearrange itself can lead to miss folding at times and may be found responsible for human disease. Therefore, studying protein domains and their role can help develop a better understanding of how diseases occur and how they can be prevented. However, studying protein domains in humans can be extremely difficult. It can be difficult due to the fact that humans have a long life span so there can be a …show more content…
Two colonies where then taken from this plate and placed into two tubes. These tubes where then taken and stored at a temperature of 37 degrees Celsius where it was able to grow overnight. After it was able to grow the plasmid DNA was isolated using Quick Lyse Minirep. The mixture was then mixed in order to resuspend the cells that had settled on the bottom and pipeted into a Quick Lyse Lysis. This tube was then Cetrifuged at 13,000 rpm and washed with a buffer. After being washed it was eluted with another buffer and set aside for the next part of the