ACTH-1-39: Product Description
ACTH 1-39 is a synthetic polypeptide hormone that consists of 39 amino acids. ACTH 1-39 is commonly referred to as Adrenocorticotropic hormone, corticotrophin, or ACTH. The molecular formula for ACTH 1-39 is C207H308N56O58S1 and a molecular mass of 4541.11 kilo Daltons. The structure of ACTH 1-39 corresponds to the full-length structure of ACTH. The natural Adrenocorticotropic hormone (ACTH) is naturally synthesized by the anterior pituitary gland and its release is stimulated by the corticotrophin-releasing hormone (CRH) which stimulates the release of corticosteroids (e.g. cortisol) from the adrenal glands. ACTH plays a major role as response to any biological stress in the hypothalamic-pituitary-adrenal axis …show more content…
In this study, the researchers sought to determine the role played by the fetal hypothalamo-pituitary axis in regulating adrenocortical growth and steroidogenesis within sheep fetus that is in late gestation period. The researchers observed a significant rise in the levels of ACTH 1-39 in the plasma between 120-125 and 136-142 days, but did not observe any change after the fetal hypothalamus and pituitary were surgically disconnected at 106-120 days of the gestation period. The results did not show the effect of both HPD and gestation age on the circulating concentrations of pro-ACTH and ACTH-containing the precursors pro-ACTH and pro-opiomelanocortin. Analysis of the fetal sheep adrenal revealed an increase in the relative abundance of mRNAs from steroidogenic enzymes CYP21A1 and CYPIIA1 during the period between 130-135 and 136-140 days of the gestation and was sustained at high levels after 141 days gestation. On the other hand, a significant increase in relative abundance of CYP17 mRNA was noted after 141 days gestation. No changes were noted in the relative abundance of the adrenal 3 beta-HSD mRNA between 130-145 days. The results indicated that the following the hypothalamo-pituitary disconnection, there was a significant decrease in the abundance of 3 beta-HSDA mRNA (by 3.1times), CYPIIA1 …show more content…
The researchers harvested 44 chambers of perifused pituitaries from mature ewes and took them through two challenges with GnRH . They then expressed their results as percentages of the baseline values obtained from each chamber. In their observations, both ACTH 1-39 and CRH did not affect tonic LH secretion compared to the controls or the first GnRH-stimulated LH secretion. In the second challenge, the response to LH to the second GnRH challenge was inhibited significantly. the two doses of ACTH 1-39 showed significant inhibition to the degree of GnRH self-priming, similarly to CRH when the researchers expressed the second LH response as a percentage of the initial LH response. The researchers concluded that both CRH and ACTH 1-39 had no effects on the secretion of LH induced by tonic, but they inhibited the secretion of LH that had been induced by GnRH by avoiding GnRH self priming. The activities of CRH can be direct or may be mediated by
ACTH 1-39 is a synthetic polypeptide hormone that consists of 39 amino acids. ACTH 1-39 is commonly referred to as Adrenocorticotropic hormone, corticotrophin, or ACTH. The molecular formula for ACTH 1-39 is C207H308N56O58S1 and a molecular mass of 4541.11 kilo Daltons. The structure of ACTH 1-39 corresponds to the full-length structure of ACTH. The natural Adrenocorticotropic hormone (ACTH) is naturally synthesized by the anterior pituitary gland and its release is stimulated by the corticotrophin-releasing hormone (CRH) which stimulates the release of corticosteroids (e.g. cortisol) from the adrenal glands. ACTH plays a major role as response to any biological stress in the hypothalamic-pituitary-adrenal axis …show more content…
In this study, the researchers sought to determine the role played by the fetal hypothalamo-pituitary axis in regulating adrenocortical growth and steroidogenesis within sheep fetus that is in late gestation period. The researchers observed a significant rise in the levels of ACTH 1-39 in the plasma between 120-125 and 136-142 days, but did not observe any change after the fetal hypothalamus and pituitary were surgically disconnected at 106-120 days of the gestation period. The results did not show the effect of both HPD and gestation age on the circulating concentrations of pro-ACTH and ACTH-containing the precursors pro-ACTH and pro-opiomelanocortin. Analysis of the fetal sheep adrenal revealed an increase in the relative abundance of mRNAs from steroidogenic enzymes CYP21A1 and CYPIIA1 during the period between 130-135 and 136-140 days of the gestation and was sustained at high levels after 141 days gestation. On the other hand, a significant increase in relative abundance of CYP17 mRNA was noted after 141 days gestation. No changes were noted in the relative abundance of the adrenal 3 beta-HSD mRNA between 130-145 days. The results indicated that the following the hypothalamo-pituitary disconnection, there was a significant decrease in the abundance of 3 beta-HSDA mRNA (by 3.1times), CYPIIA1 …show more content…
The researchers harvested 44 chambers of perifused pituitaries from mature ewes and took them through two challenges with GnRH . They then expressed their results as percentages of the baseline values obtained from each chamber. In their observations, both ACTH 1-39 and CRH did not affect tonic LH secretion compared to the controls or the first GnRH-stimulated LH secretion. In the second challenge, the response to LH to the second GnRH challenge was inhibited significantly. the two doses of ACTH 1-39 showed significant inhibition to the degree of GnRH self-priming, similarly to CRH when the researchers expressed the second LH response as a percentage of the initial LH response. The researchers concluded that both CRH and ACTH 1-39 had no effects on the secretion of LH induced by tonic, but they inhibited the secretion of LH that had been induced by GnRH by avoiding GnRH self priming. The activities of CRH can be direct or may be mediated by