Pglo Lab Report

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In this experiment, the hypothesis gave the expected results. The positive DNA has pGLO that can grow regardless that has the presence of the Ampicillin. The negative DNA does not have pGLO and thus cannot grow in the presence of Ampicillin because pGLO contains ampicillin-resistance gene. When it is not pGLO breaks the Ampicillin. The LB does not have Ampicillin and can grow colonies on the agar plates. For example, in the LB (- DNA) shown large number of white because contain negative DNA but it is present LB. In the LB/Amp (pGLO negative) shown any growth colony because it is presents Ampicillin that break the bacterial cells. In the LB/Amp (pGLO positive), shown growth colony regardless this has Ampicillin. The reason in this case, it is because in the positive DNA has pGLO that can grow bacteria colony on the agar plate. …show more content…
The LB/Amp/Ara (pGLO positive) shown growth colonies on the agar plate. The brilliant green color shown in this plate is called GFP (Green Fluorescent Protein). The Green Fluorescent Protein is expressed only in the presence of Arabinose (Ara). The GFP is expressed after transformation such as a brilliant green color when it was seen in the UV light. The transformation efficiency describes how effective was the transformation of the pGLO added into the E. coli bacteria. The transformation efficiency should be a very high number of transformation efficiency. In this experiment, the transformation efficiency are the followings: LB/Amp (+DNA) was 20307.69 and the LB/Amp/Ara (+DNA) was 11538.46 of transformation efficiency, that means they are a good transformation efficiency because both are a very high number putting one microgram of pGLO into the

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