The reasons why kidney and skeletal muscle chosen for the kinetic analysis of LDH:
Lactate dehydrogenase (LDH) is the enzyme that coverts high level lactate to pyruvate and maintain equilibrium level in skeletal muscle. In the abive case Lactate dehydrogenase action is completely correlated to the muscular fatigue. The production of lactic acid during severe excerise or heavy work load is leading to muscle fatigue. Normally at rest means the excess lactate converted to pyruvate . Usually LDH reduces lactate level by various ways by generating NAD+ with glyceradehyde -3-phophate and also by promoting ATP energy production through second period of glycolytic phase to the muscle cells under heavy work loads in skeletal muscle.
Inorder to maintain homeostasis and get back normal blood glucose levels, renal tubular filration of glucose must the intensed thereby kidney elimination of glucose reduces to bring the PH to normal as lactic acid has the ability to accumulate more H+ ions. These H+ ions must be …show more content…
It also depends upon the regulatory substances such as substrate levels or cofactors during the conversion of pyruvate to lactate and vice versa whereas LDH elasticity coefficient complexly relies upon the multistep pathways associated predominantly by regulatory molecules and associated substrate concentration.
Monocarboxylate transporter 2- lactate shuttle : It is LDH transporter playing a predominant role where it usually transport pyruvate via peroxisomes further degraded by LDH into lactate associated conversion of NADH to NAD+ for beta oxidation of fatty acids.
Viversa, lactate produce in the above reaction will be trasported back via membrane monocarboxylate transporter 2 further undergoes oxidation and will be finally converted to pyruvate by cytoplasmic LDH leaing to reproduction of NADH to produce
Lactate dehydrogenase (LDH) is the enzyme that coverts high level lactate to pyruvate and maintain equilibrium level in skeletal muscle. In the abive case Lactate dehydrogenase action is completely correlated to the muscular fatigue. The production of lactic acid during severe excerise or heavy work load is leading to muscle fatigue. Normally at rest means the excess lactate converted to pyruvate . Usually LDH reduces lactate level by various ways by generating NAD+ with glyceradehyde -3-phophate and also by promoting ATP energy production through second period of glycolytic phase to the muscle cells under heavy work loads in skeletal muscle.
Inorder to maintain homeostasis and get back normal blood glucose levels, renal tubular filration of glucose must the intensed thereby kidney elimination of glucose reduces to bring the PH to normal as lactic acid has the ability to accumulate more H+ ions. These H+ ions must be …show more content…
It also depends upon the regulatory substances such as substrate levels or cofactors during the conversion of pyruvate to lactate and vice versa whereas LDH elasticity coefficient complexly relies upon the multistep pathways associated predominantly by regulatory molecules and associated substrate concentration.
Monocarboxylate transporter 2- lactate shuttle : It is LDH transporter playing a predominant role where it usually transport pyruvate via peroxisomes further degraded by LDH into lactate associated conversion of NADH to NAD+ for beta oxidation of fatty acids.
Viversa, lactate produce in the above reaction will be trasported back via membrane monocarboxylate transporter 2 further undergoes oxidation and will be finally converted to pyruvate by cytoplasmic LDH leaing to reproduction of NADH to produce