CX = A1 / ax1 ………………..... (1)
CY = [A2 – (ax2 × CX)] / ay2…...(2)
Where,
A1 and A2are absorbance of mixture at λ1 and λ2respectively.
ax1 and ax2 are absorptivities of DCL at λ1 and λ2, respectively,
ay1 and ay2 are absorptivities of SER at λ1 and λ2, respectively,
CXandCYare concentrations of DCL and SER, respectively.
Concentration of DCL and SER in the powder mixture is found by using equation (3) and (4).
CDCL = A1/ 20.14 ………………………… (3)
CSER = [A2 – (43.08×CDCL)]/14.28 … (4)
Where,
20.14 and 43.08 are absorptivities of DCL at λ1 and λ2 respectively.
0.00 and 14.28 are absorptivities of SER at λ1 and λ2 respectively. …show more content…
No. Amount Present in µg/ml Amount Found in µg/ml Amount Found in % SER DCL SER DCL SER DCL
1. 10 50 O9.90 50.00 99.00 100.00
2. 10 50 09.87 50.20 98.70 100.40
3. 10 50 10.00 49.90 100.00 101.80
4. 10 50 09.90 50.20 99.00 100.40
5. 10 50 10.10 50.00 101.00 100.00
6. 10 50 09.87 50.10 98.70 100.20
Table 3: Statistical Validation of Pure Drugs
Drug Mean*
(%) Standard Deviation* Co-efficient of Variation* (% R.S.D.) Standard Error*
SER 99.40 0.9186 0.9241 0.3773
DCL 100.46 0.6772 0.6740 0.2752
SER is Serratiopeptidase and DCL is Diclofenac potassium,* Here Mean is the average of (n=6) results.
Table 4: Data forAnalysis of Tablet
Sr.
No. Label Claim (mg/tab) Amount Found (mg/tab) % of Label Claim SER DCL SER DCL SER DCL
1. 10 50 O9.70 49.40 97.00 98.80
2. 10 50 09.87 50.20 98.70