Firstly, 60 mL of silica was added to a 250 mL beaker. After which, an approximate 100 mL of chloroform was added to the beaker to make a pourable slurry. The slurry was then poured into the column and the solvent just about the silica was drained off into a conical flask. To minimize losses, the silica slurry beaker was rinsed with more chloroform and added to the column. Next, a thin layer of acid washed sand (3mm – 4mm) was added to the column. Next, two ‘curcumin soft gels’ was puncture using a scissor and spatula. The soft gels were squeezed and added to a 50 ml beaker with 30 ml of chloroform. The mixture was mixed well and the extract solution was carefully transferred to the top of the column using a Pasteur pipette. The column was then drained to allow the solvent level to reach the top of the silica gel. Afterwards, a solution of 0.3% Methanol in chloroform was carefully added to the top of the column to elute the column. Upon adding the fresh eluent, curcumin and other compounds were eluted at a different rate. The eluted compounds were collected using test tubes. The collection of eluted compounds was stopped when the first intense band had reached the bottom of the column. At the meantime, TLC was carried out for all eluent collected from the column. Subsequently, a clean, dry round bottom flask was weighed and the column eluents (sample 7 – 11) with one ink dot was added to the round bottom flask. The flask was put into a rotary evaporator to remove all the chloroform. After removing the chloroform, the flask was placed in the oven for 15 minutes. The flask was re-weighed after the solvent had
Firstly, 60 mL of silica was added to a 250 mL beaker. After which, an approximate 100 mL of chloroform was added to the beaker to make a pourable slurry. The slurry was then poured into the column and the solvent just about the silica was drained off into a conical flask. To minimize losses, the silica slurry beaker was rinsed with more chloroform and added to the column. Next, a thin layer of acid washed sand (3mm – 4mm) was added to the column. Next, two ‘curcumin soft gels’ was puncture using a scissor and spatula. The soft gels were squeezed and added to a 50 ml beaker with 30 ml of chloroform. The mixture was mixed well and the extract solution was carefully transferred to the top of the column using a Pasteur pipette. The column was then drained to allow the solvent level to reach the top of the silica gel. Afterwards, a solution of 0.3% Methanol in chloroform was carefully added to the top of the column to elute the column. Upon adding the fresh eluent, curcumin and other compounds were eluted at a different rate. The eluted compounds were collected using test tubes. The collection of eluted compounds was stopped when the first intense band had reached the bottom of the column. At the meantime, TLC was carried out for all eluent collected from the column. Subsequently, a clean, dry round bottom flask was weighed and the column eluents (sample 7 – 11) with one ink dot was added to the round bottom flask. The flask was put into a rotary evaporator to remove all the chloroform. After removing the chloroform, the flask was placed in the oven for 15 minutes. The flask was re-weighed after the solvent had