The results found in the collated table supports the hypothesis, however, it was not as accurate as expected. From observation of the collated results, it can be seen that the greatest froth volume is found at 30oC, rather than the predicted optimum temperature of 39oC. Errors within the individual group results, concluded that the both 10oC and 20oC were wrong due to the vast froth volumes. For a smaller amount of catalase, this would result in a smaller froth volume, conversely, with a greater amount of catalase, the froth volume will be greater. Overall, the results found in the combined results did support the hypothesis, but they were not as accurate as predicted.
From the results obtained within the collated results, it can be concluded …show more content…
As the temperature increases, so does the rate of reaction accordingly, but extreme temperatures denature enzymes. (BBC, 2016) As it can be seen in figure 2, the greater the temperature, the greater the kinetic energy, thus causing more collisions between the enzyme and the substrate. More collisions, means the substrate and the enzyme are binding more often, causing the reaction to occur at a much faster rate. Until the point at which it temperature affects the active site of the enzyme, causing it to denature. The chemical formula for the catalase in the reaction is 2(H2O2) ▻ 2H2O + O2. As is it can be seen, the two products of the reaction is water and oxygen, thus why there is froth when the hydrogen peroxide and catalase are mixed. Overall, it can be concluded that the increasing temperature increases the reaction rate between the catalase and the hydrogen peroxide/detergent …show more content…
These limitations were caused overall due to errors with the apparatus used. For the experiment, the maximum temperature that was tested was 50oC, this is due to the potential of breaking the test tubes due to the extreme heat. The use of the same brand of thermometer to avoid error between the manufacturing of the thermometer could have produced more reliable and valid results. Each group should have tested the equipment before hand to see whether there was any differences with the thermometer and timer. Furthermore, a limitation within the experiment is the testing the temperatures in increments of 10oC, making it hard to observe the optimum temperature of the catalase. Lastly, the source of catalase is a limitation as each group may not have had the same concentration due to the unequal densities in the mixture of detergent and hydrogen
From the results obtained within the collated results, it can be concluded …show more content…
As the temperature increases, so does the rate of reaction accordingly, but extreme temperatures denature enzymes. (BBC, 2016) As it can be seen in figure 2, the greater the temperature, the greater the kinetic energy, thus causing more collisions between the enzyme and the substrate. More collisions, means the substrate and the enzyme are binding more often, causing the reaction to occur at a much faster rate. Until the point at which it temperature affects the active site of the enzyme, causing it to denature. The chemical formula for the catalase in the reaction is 2(H2O2) ▻ 2H2O + O2. As is it can be seen, the two products of the reaction is water and oxygen, thus why there is froth when the hydrogen peroxide and catalase are mixed. Overall, it can be concluded that the increasing temperature increases the reaction rate between the catalase and the hydrogen peroxide/detergent …show more content…
These limitations were caused overall due to errors with the apparatus used. For the experiment, the maximum temperature that was tested was 50oC, this is due to the potential of breaking the test tubes due to the extreme heat. The use of the same brand of thermometer to avoid error between the manufacturing of the thermometer could have produced more reliable and valid results. Each group should have tested the equipment before hand to see whether there was any differences with the thermometer and timer. Furthermore, a limitation within the experiment is the testing the temperatures in increments of 10oC, making it hard to observe the optimum temperature of the catalase. Lastly, the source of catalase is a limitation as each group may not have had the same concentration due to the unequal densities in the mixture of detergent and hydrogen