Amylase's Role In Bread Processing Case Study

Improved Essays
2.4 Amylase’s Role in Bread Processing Bread making process is different from one company and the other company. There are many uniqueness of formula from each company but generally, the process is divided into three processes. The first step is dough mixing. The starch inside the flour can be broken by the alpha-amylase and will produce dissolved amylose which is the enzyme substrate to the next amylose degradation process. Starch hydrolysis process has a very important role to dough’s rheology characteristic because amount of water will be bonded by the hydrolyzed starch. In dough mixing process there will be an intensive mass transfer. Contact between enzyme and substrate can go well and it will producing simple sugars such as glucose …show more content…
This dough fermentation is defined as amino acid decomposition process in anaerobic way, which means it does not need any oxygen. Compounds that can be parsed in fermentation process is carbohydrate that have been breakdown into simple sugars, for example starch hydrolysis into glucose units. Flour based ingredients if it is mixed together, and then starch and protein from the flour will absorb water and form dough and yeast will do the fermentation to sugar that exist and produce carbon dioxide (CO2). Fermentation dough is shown with the dough will expands because of the formation of carbon dioxide that be detained inside the dough. Maltose and glucose is very important as substrate for yeast in dough fermentation process. The addition of glucoamylase it can increasing and fastening the glucose production and it will be used by yeast. Beside then, the addition of glucoamylase will activate yeast and fastening fermentation process or dough …show more content…
At this process, several amount of water will disappear, yeast will not be formed, starch will gelatinize and protein will agglomerate. Because of these things, it will gives a stable form for the bread. In the starting process of roasting, dough’s viscosity is decreasing and the enzyme activity will increase. When it reaches 56˚C, starch’s gelatination is happening and amylolysis reaction will go easier. Optimal temperature of enzyme activity and damage because of heat is varies. Starch hydrolysis that is gelatinize will form dextrin and simple sugars, also in the same time water release happened. This process will lead to crumb stickiness and increase the intensity of crust color. Bread’s color is the result from the Maillard reaction, because of that oligosaccharide concentration increase and simple sugars that produced from the glucoamylase activity also resulting of increase browning

Related Documents

  • Improved Essays

    Step 1. Make Drosophila culture medium First, to prepare a culture medium, four plastic vials were obtained and labeled with numbers 1 – 4. In each of the four vials, two teaspoons of medium mixture were added.…

    • 731 Words
    • 3 Pages
    Improved Essays
  • Great Essays

    Amylase Experiment

    • 1123 Words
    • 5 Pages

    By adding more temperatures, the results become more precise as to what temperature amylase denatures at, and what temperature is the most efficient for amylase. Variations that differ from the variables in this experiment could be the effect of pH levels on enzyme activity, which would show the best pH levels for amylase to be most efficient and whether it works best at low, medium or high pH levels. The concentration of amylase would also be a variation that would show whether amylase works faster if there is more of it or not. If all of these variations were done, it would show in what conditions amylase works best at, and if the human body fits those conditions or…

    • 1123 Words
    • 5 Pages
    Great Essays
  • Superior Essays

    Cellular Respiration Lab

    • 1244 Words
    • 5 Pages

    There are enzymes that are not functioning correctly before these compounds in which they are accumulating. In strain 136, the compound Fructose 1,6 biphosphate is collected in experiment 1 and in experiment two we see that it grew in restrictive temperature where it is also adapting to other food sources. The enzyme that is mutated to get use to its restrictive temperature is phosphofructokinase and this enzyme make its phosphate group go to the other end of the sugar. In strain 152, 3-phosphoglycerate accumulates because of the enzyme Phosphoglycerokinase helps it to grow in the restrictive temperature. The pyruvate kinase is an enzyme that is functioning incorrectly after because in the second experiment pyruvate did not accumulate and it did not grow in the unknown food source and restrictive temperature.…

    • 1244 Words
    • 5 Pages
    Superior Essays
  • Superior Essays

    Amylase Biology Lab Report

    • 2820 Words
    • 12 Pages

    These amylases are to be tried at various temperatures fluctuating from 0° C to 80° C noted between 2 minute intervals until time extents to 10 minutes. This experiment will help define optimal temperatures of the bacterial amylase and the fungal amylase. It is predicted that at temperature of 55° C bacterial amylase will have its optimal temperature considering that bacteria number increases in warmer places. Furthermore, when the temperature is at 40° C, fungal amylase will have its optimal temperature considering that fungi thrive in cooler places. Additional matters to be attended in the following laboratory experiment are the denaturation of enzymes at a certain temperatures and enzyme activity as temperatures increase or decrease in relation to the optimal…

    • 2820 Words
    • 12 Pages
    Superior Essays
  • Decent Essays

    The enzyme (Sternzym VC 5000) was obtained from SternEnzym company. Sternzym VC 5000 contains a pure fungal α-amylase without detrimental side activities. The enzyme has a standardized activity providing constant properties, and its fine granulation ensures an uniform distribution in flour, dry premixes or dough [32]. The activity of this fungal α-amylase is 5,000 SKB/g with optimum temperature range from 30 ℃ to 55 ℃ and optimum pH value range from 4 to 6.5.…

    • 147 Words
    • 1 Pages
    Decent Essays
  • Decent Essays

    The enzymes in the the denatured pineapple allowed the Jell-O’s proteins to function and structure properly. However the natural pineapple didn’t allow the Jell-O to structure and function properly. The acting substrate in the experiment was the Jell-O’s proteins.…

    • 115 Words
    • 1 Pages
    Decent Essays
  • Superior Essays

    Yeast Enzyme Lab Report

    • 647 Words
    • 3 Pages

    Visual bubbling occurred in each sample with fewer bubbles at 46 and 53° C. A 54% decrease in enzyme activity was seen between control and highest temperature of 53° C. The highest enzyme activity was seen at 32°C, which…

    • 647 Words
    • 3 Pages
    Superior Essays
  • Decent Essays

    Once the amylase breaks down the starch in a food the iodine solution would turn clear. We decided to use a potato, a very starchy food, to represent the breakdown. We figured out different ways to increase the rate of the breakdown of amylase and a potato and then compare the rates of those breakdowns to the initial rate of breakdown…

    • 244 Words
    • 1 Pages
    Decent Essays
  • Improved Essays

    Amylase Lab

    • 469 Words
    • 2 Pages

    Amylase is an enzyme that is specifically in charge of breaking down starches into sugar by using water (hydrolysis). In general amylase is present in all living organisms, but can vary between different species. Porcine amylases and the amylases that is found in humans has an 81.7% similarity (Argwall, 1987). In order to determine the optimal environmental conditions of porcine liver amylase the experiments conducted in this lab will test ph. (acidity) and the temperature. Each variable will be tested individually by gradually increasing both during each trail.…

    • 469 Words
    • 2 Pages
    Improved Essays
  • Improved Essays

    The versatility and easy production of bread is what allows so many variations such as pancakes, muffins, hamburger buns, and French bread to appear because the availability of bread allows experiments and tests to happen which leads to production of a new kind of bread. Overall bread plays a big part in the life of humanity since bread is one the main foods people rely on in their diet throughout time and because of the bond between bread and humans it allows bread to integrate into the daily lives of…

    • 1160 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Part B Two test tubes were labeled, one with “yeast” and the other with “water”. 1 ml of yeast suspension and 4 ml of distilled water was added to the “yeast’ labeled tube. 5 ml of distilled water was added to the “water” tube. 5 ml of sucrose solution was added to each test tube and stirred.…

    • 1180 Words
    • 5 Pages
    Improved Essays
  • Superior Essays

    Introduction Debates concerning the health effects of artificial sweeteners are relevant not only for scientists and dieticians, but also for diabetics and individuals who are trying to lose weight. According to the American Diabetes Association (2014), aspartame (Equal, NutraSweet); acesulfame potassium (Sunett, Sweet One); neotame; sucralose (Splenda); saccharin (Sweet ‘N Low, Sweet Twin, Sugar Twin); and advantame are six alternatives to natural sugar that are commonly added to foods and incorporated into store-bought foods. Rebaudioside A (Stevia) is a natural sweetener that is also quite common (para. 3, 5). The benefits of artificial sweeteners include their concentrated flavor and their low calorie content.…

    • 1366 Words
    • 6 Pages
    Superior Essays
  • Decent Essays

    Arrowroot’s Root Arrowroot starch is easily digested and because of that it is a good ingredient for baby’s food and people with dietary restrictions’ food. Arrowroot starch can also be used as food for children with autism and diet food for elderly as well as patients who still in recovery period (Ariesta , 2004). One of the food products made from arrowroot starch is baby biscuits. In the making process of baby biscuit, starch will undergo a process called gelatinization. Arrowroot starch will be gelatinized at the temperature 63, 96°C – 70°C.…

    • 414 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    Amylase is an enzyme that breaks down starch. Among other factors, high temperatures denature enzymes. Through experimentation this was not observed. The results of the experiment resulted in flawed data. By human error, a mistake was made that affected how well the enzymes synthesized starch.…

    • 691 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Food Digestion Essay

    • 845 Words
    • 4 Pages

    Digestion can be described as food being broken down into its most basic and simple components so that we can absorb the nutrients to give us energy and nutrition. Digestion occurs in what is known as the gastrointestinal (GI) tract which consists of the mouth, esophagus, stomach, small intestine, large intestine (colon), and other important organs such as salivary glands, the liver, pancreas, and gallbladder. Digestion first occurs with food entering the mouth. Food is usually comprised of three macronutrients: carbohydrates, fats (lipids), and proteins.…

    • 845 Words
    • 4 Pages
    Improved Essays